Quality Control of Next|Generation Sequencing|Based HIV|1 Drug ...

Sensitive HIV-1 DNA Pol Next-Generation Sequencing for the ...

Next-generation sequencing (NGS) offers increased sensitivity for characterising archived drug resistance mutations (DRMs) in HIV-1 DNA for ...

HIV-1 Drug Resistance Assay Using Ion Torrent Next Generation ...

HIV-1 antiretroviral therapy management requires sequencing the protease, reverse transcriptase, and integrase portions of the HIV-1 pol ...

Next Generation Sequencing of HIV-1 Drug Resistant Mutations V.2

There is also the potential that this protocol can be expanded to whole genome sequencing for HIV-1. The imminent sunsetting of ViroSeq (Abbott ...

External Quality Assessment Program for Next-Generation ...

Next-Generation Sequencing-Based HIV Drug. Resistance Testing ... Quality control trial for human immunodeficiency virus type 1 drug.

External Quality Assessment Program for Next-Generation ... - OUCI

Next-generation sequencing (NGS) is likely to become the new standard method for HIV drug resistance (HIVDR) genotyping. Despite the significant advances in ...

Implementation of Next-Generation Sequencing-Based HIV Drug ...

The implementation of NGS-based HIV drug resistance profiling represents a significant advancement in the management of HIV infection. By ...

Multi-Laboratory Comparison of Next-Generation to Sanger-Based ...

Understanding how variation in NGS methods influences sequence quality is essential for NGS-based HIV-1 drug resistance genotyping. Keywords: ...

A MiSeq-HyDRA platform for enhanced HIV drug resistance ... - Nature

Here we present a next generation sequencing (NGS)-based HIVDR typing platform that leverages the advantages of Illumina MiSeq and HyDRA Web.

Universal Amplification, Next-Generation Sequencing, and ...

Furthermore, we used our method to investigate mutations associated with HIV-1 drug resistance in clinical samples at the level of the complete genome. Drug ...

In-house NGS-Based HIV Genotyping protocol v.01

The assay utilizes next-generation sequencing (NGS) technology to identify mutations associated with antiretroviral treatment (ART) resistance ...

MicroArray/Sequencing Quality Control (MAQC/SEQC) - FDA

... next-generation sequencing (NGS) methodologies in regulatory settings and precision medicine. Specifically, SEQC2 focused on 1) assessing ...

Validation of an HIV whole genome sequencing method for HIV drug ...

In 2022, our clinical microbiology laboratory sought to validate a next-generation sequencing (NGS)-based HIVDR assay to replace the previous ...

The use of next-generation sequencing for the surveillance of drug ...

Validating NGS protocols and embedding strong quality assurance (QA) mechanisms at each step. • Building capacity among laboratory staff, bioinformatics staff, ...

Human Immunodeficiency Virus 1 Drug Resistance by Next ...

The protease gene, integrase gene and the reverse transcriptase gene of the viral genome are sequenced using Next Generation Sequencing. Drug resistance is ...

Targeted next-generation sequencing to diagnose drug-resistant ...

Targeted next-generation sequencing (NGS) can rapidly and simultaneously detect mutations associated with resistance to tuberculosis drugs across multiple gene ...

First evaluation of the Next-Generation Sequencing platform ... - PLOS

The WHO urges action against the threat posed by HIV drug resistance. It is well known that the sensitivity of Next-Generation Sequencing (NGS) ...

Application of high-throughput sequencing technology in HIV drug ...

Van Laethem,. Quality control of next-generation sequencing-based HIV-1 drug resistance data in clinical laboratory information systems framework, Viruses 12 ...

A Solution for HIV Drug Resistance Genotyping With Exatype, End to ...

... Based Sequence Analysis and Patient HIV Drug Resistance Result Generation ... sequence editing, quality assurance and mutation scoring using different software.

Performance comparison of next generation sequencing analysis ...

Performance comparison of next generation sequencing analysis pipelines for HIV-1 drug resistance testing. ... quality control strategies. Findings from ...

Evaluation of the ABL NGS assay for HIV-1 drug resistance testing

The NGS assay's unit cost fell rapidly as the number of samples per run increased. Compared with Sanger sequencing, the ABL NGS HIV genotyping ...