- Role of High|Fidelity Escherichia coli DNA Polymerase I in ...🔍
- A mutant of Escherichia coli K12 deficient in the 5′–3🔍
- The role of replication clamp|loader protein HolC of Escherichia coli ...🔍
- In Vivo Mutagenesis by Escherichia coli DNA Polymerase I🔍
- Major Molecular Events of DNA Replication🔍
- DNA Polymerase I🔍
- Structure of the SSB–DNA polymerase III interface and its role in ...🔍
- Basic Mechanisms of Replication🔍
coli strain that lacked DNA polymerase I 3
Role of High-Fidelity Escherichia coli DNA Polymerase I in ...
In addition, cellular studies were conducted using E. coli strains that were either wild type or deficient in all three DNA damage-inducible polymerases, i.e., ...
A mutant of Escherichia coli K12 deficient in the 5′–3 - SpringerLink
A new mutation affecting DNA polymerase I of Escherichia coli is described. Strains carrying mutation polA107 are similar to polA1 strains in their ...
The role of replication clamp-loader protein HolC of Escherichia coli ...
In Escherichia coli, DNA replication is catalyzed by an assembly of proteins, the DNA polymerase III holoenzyme. This complex includes the ...
In Vivo Mutagenesis by Escherichia coli DNA Polymerase I
The crystal structure of the Klenow fragment of pol I (which lacks the 5⬘–3⬘ exonuclease domain) reveals an architecture that is common among DNA polymerases ...
Major Molecular Events of DNA Replication - Nature
A third family of proteins, the topoisomerases, reduce some of the torsional strain caused by the unwinding of the double helix. A schematic diagram shows three ...
DNA Polymerase I - Worthington Enzyme Manual
coli strain with a mutation that affected the DNA polymerase and surprisingly found that the mutant synthesized DNA normally. This discovery cast doubts on ...
Structure of the SSB–DNA polymerase III interface and its role in ...
In Escherichia coli, SSB's association with the χ subunit of the DNA polymerase III holoenzyme has been proposed to confer stability to the replisome.
Basic Mechanisms of Replication
coli DNA polymerase I lacks the 5' to 3' exonuclease, so the 3-D structure ... coli strain with a mutation affecting DNA polymerase. Nature 224:1164 ...
Chapter 9: DNA Replication - Chemistry - Western Oregon University
coli has a total of five DNA polymerases. Three of these enzymes are involved in DNA replication (DNA polymerases I, II, and III). DNA polymerase III is the ...
DNA Polymerase I Large (Klenow) Fragment - Promega Corporation
DNA Polymerase I Large (Klenow) Fragment is a 68kDa C-terminal fragment of E. coli DNA Polymerase I that lacks the 5´→3´ exonuclease activity of intact DNA ...
Dpb2 Integrates the Leading-Strand DNA Polymerase into the ...
In E.coli, replisome assembly results from the association at. DNA replication forks of the clamp loader with DNA helicase and three identical DNA polymerase ...
If Polymerase III still replicates DNA from 5' to 3' on the lagging ...
DNA polymerase III holoenzyme is the enzyme primarily responsible for replicative DNA synthesis in E. coli. It carries out primer-initiated 5' ...
Lack of the H-NS Protein Results in Extended and Aberrantly ...
Lack of the H-NS Protein Results in Extended and Aberrantly Positioned DNA during Chromosome Replication and Segregation in Escherichia coli. Authors: Emily ...
Replication of the Escherichia coli Chromosome with a Soluble ...
coli DNA polymerase I (EC 2.7.7.7) and DNA ligase (EC. 6.5.1.2). Study of the ... coli strain H560 (polA 1-, endo 1-, thy-) was used for the preparation ...
Solved Mutations in pol I that completely lack the 5' --> 3' | Chegg.com
coli strain with a mutant poll that lacks the 5' --> 3' exonuclease activity because the bacteria always die. The mutant pol I cannot remove the RNA primers ...
A single‐molecule approach to DNA replication in Escherichia coli ...
We also found the average speed of the replication fork to be only 264 ± 9 nt s−1 in a dnaE173-eCOMB strain producing a mutant-type of the ...
DNA Polymerase I, Large (Klenow) Fragment - NEB
An E. coli strain that contains the E. coli polA gene that has had its 5'→3' exonuclease domain removed. This product is related to ...
Fidelity and Error Specificity of the a Catalytic Subunit of Escherichia ...
coli DNA polymerase III holoenzyme. From an overproducing strain, a subunit was purified essentially free of 39 exonuclease activity. The current ...
Single-molecule visualization of fast polymerase turnover in ... - eLife
The Escherichia coli DNA replication machinery has been used as a road map to uncover design rules that enable DNA duplication with high ...
DNA polymerase adds nucleotides to the three prime (3')-end of a DNA strand, one nucleotide at a time. Every time a cell divides, DNA polymerases are required ...