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A guide to choosing the right super|resolution microscopy technique


Beyond the Limit: The World of Super-Resolution Microscopy

When selecting between different super-resolution tools and techniques, it is important to ask a number of questions to determine the best ...

Technological advances in super-resolution microscopy to study ...

In addition, we discuss the pluses and minuses of each technology to guide the reader in choosing the right method. We also provide guidance in ...

The cell biologist's guide to super-resolution microscopy

(Lambert, 2019) and the microscope to be used. • The speed of acquisition needs to be taken into consideration when choosing the most appropriate SRM technique.

A beginner's guide to improving image acquisition in fluorescence ...

Best practices for using microscope cameras ... Although the ideal image acquisition settings vary depending on the application and sample, two ...

Choosing the Right Microscope - Buying Guides MedicalExpo

Scanning near-field optical microscopy (SNOM): this is a super-resolution microscopy technique. It makes it possible to exceed the Abbe limit by detecting ...

[E-Book] Super-Resolution Microscopy - Bruker

With this enhanced resolving power, super-resolution microscopy methods allow the imaging and quantitative analysis of cellular dynamics and nanostructures that ...

Imaging cellular structures in super-resolution with SIM, STED and ...

Many biological questions require fluorescence microscopy with a resolution beyond the diffraction limit of light. Super-resolution methods ...

Single-Molecule Super-Resolution Imaging | Nikon's MicroscopyU

Stochastic optical reconstruction microscopy (STORM) is a single-molecule superresolution technique that is capable of providing resolutions down to 10 ...

Quantitative Single-Molecule Localization Microscopy

Seeing beyond the limit: a guide to choosing the right super-resolution microscopy technique. J. Biol. Chem. 297:100791. [Google Scholar]. 174 ...

Super-Resolution Microscopy with Dense Grid After Interpolation

The conventional camera image's pixel size of super-resolution (SR) microscopy is almost the point spread function's standard deviation, and the grid of a ...

Tutorial: guidance for quantitative confocal microscopy - SCIAN-Lab

more detail below in 'Choosing the right microscope'. ... FPs is particularly useful for super-resolution microscopy techniques14 but can also be applied for ...

Live-Cell Imaging: Choosing the Right Technique - Bitesize Bio

One of the main advantages of confocal laser scanning microscopy (CLSM) is the ability to eliminate out-of-focus light and information. It is also possible to ...

How Olympus Super Resolution and Spinning Disk Technology ...

Therefore, a confocal fluorescence microscope cannot be used as a super resolution microscope. Figure1.Schematic presentation of two-point resolution. Figure1.

How to choose the right m icroscopic technique? - SIMTRUM.com

This technique requires an epifluorescence microscope and a motorized z-axis stepper to take images of different z sections through the specimen. Software is ...

Navigating the Challenges and Future of Super-resolution Microscopy

General Guide to Choosing Appropriate SRM. While all SRM techniques provide resolutions beyond the diffraction limit, each has unique ...

Comparing Super-Resolution Microscopy Techniques to Analyze ...

Instead, super-resolution by stimulated emission depletion (STED) microscopy achieving a resolution of ~50 nm laterally and ~130 nm axially has not yet ...

An introduction to optical super-resolution microscopy for the ...

Even though some of the fundamental concepts enabling super-resolution light microscopy have been known for quite some time, practically feasible ...

A comparison of super-resolution microscopy techniques for ...

This study compared five fluorescence microscopy methods, including confocal and STED microscopy, to determine their effectiveness in resolving ...

Universal PAINT – Dynamic Super-Resolution Microscopy

Super-resolution microscopy techniques have revolutionized biology for the last ten years. With their help cellular components can now be ...

Microscopy Resources - West Campus Imaging Core - Yale University

Super-resolution. The cell biologist's guide to super-resolution microscopy - Journal of Cell Science, 2020. Image Analysis and Digital Imaging. Digital Images ...