Development of a multiplex real|time quantitative reverse ...

The Basics: RT-PCR | Thermo Fisher Scientific - US

Why Real-Time RT-PCR? Real-Time PCR Chemistries; Quantitation of Results ... The Invitrogen™ QuantumRNA™ Technology in Multiplex Quantitative RT-PCR using 18S ...

Clinical evaluation of a panel of multiplex quantitative real-time ...

We developed a panel of multiplex quantitative real-time reverse transcription polymerase chain reaction (mqRT-PCR) assay consisting of ...

Lab Protocol Paper: Use of a High-throughput, Multiplex Reverse ...

We developed a high-throughput, multiplex reverse-transcription quantitative ... , et al. Development and evaluation of a 'real-time' RT-PCR for ...

Development and validation of a multiplex real-time qPCR assay ...

Quantitative PCR methods for detecting bacterial DNA are more sensitive and could aid in differentially diagnosing leprosy from other dermatological conditions.

Establishment and evaluation of a multiplex real-time RT-PCR for ...

This study sought to establish a real-time reverse transcription (RT)-PCR method to differentially detect canine distemper virus (CDV) ...

Multiplex Analyses Using Real-Time Quantitative PCR. - Abstract

Quantitative polymerase chain reaction (qPCR) is a routinely used method for the detection and quantitation of gene expression in real time. Multiplex qPCR ...

Quantitative real-time RT-PCR – a perspective in

The real-time reverse transcription polymerase chain reaction (RT-PCR) uses fluorescent reporter molecules to monitor the production of amplification products.

Development and Application of a Multiplex Reverse-Transcription ...

Characterization of the mixture of genotypes of a Citrus tristeza virus isolate by reverse transcription-quantitative real-time PCR. J. Virol. Methods 164 ...

Deducing Multidecadal Anthropogenic Global Warming Trends ...

Quantitative Multiplex Real-Time Reverse Transcriptase–Polymerase Chain Reaction with Fluorescent Probe Detection of Killer Immunoglobulin-Like Receptors ...

Development of a New Multiplex Real-Time RT-PCR Assay for ...

In this one-step, real-time qualitative RT-PCR assay, a TaqPath 1 ... reverse primer at 25 μmol/L, 0.16 μL of S gene probe at 25 μmol/L ...

Development of a multiplex real-time reverse transcriptase ...

Development of a multiplex real-time reverse transcriptase-polymerase chain reaction for equine infectious anemia virus (EIAV). Cook, RF; · ...

Development of a Multiplex Real-Time Quantitative PCR Assay for ...

Real-time quantitative polymerase chain reaction. (qPCR) has revealed various dominant BV-related bacteria. G. vaginalis and A. vaginae were the ...

Improvement and Optimization of a Multiplex Real-Time Reverse ...

7,20 In recent years, a number of rapid diagnostic tests for VSV have been developed,1,4–6,17 including different real-time reverse transcription polymer- ase ...

Real-time polymerase chain reaction - Wikipedia

A real-time polymerase chain reaction (real-time PCR, or qPCR when used quantitatively) is a laboratory technique of molecular biology based on the polymerase ...

Development of a multiplex real-time reverse transcriptase ...

Fully quantitative methods to determine viral. RNA levels in EIAV infected animals have been based on the principle of competitive RT-PCR. (Piatak et al., 1993) ...

Quantitative PCR Basics - Sigma-Aldrich

Quantitative PCR (formally quantitative real-time PCR, qPCR) detection ... quantification of multiple targets in a single (multiplex) reaction. A ...

Quantitative Multiplex Real-Time Reverse Transcriptase ... - ProQuest

We developed an effective method to determine KIR3DL3/KIR2DL4 expressions based on a multiplex quantitative real-time Reverse transcription polymerase chain ...

Development of multiplex real-time PCR for simultaneous detection ...

Background Fungi are common opportunistic pathogens that pose a significant threat to immunocompromised patients, particularly when late ...

A multiplex real-time reverse transcription polymerase chain ... - OUCI

... developed a multiplex real-time quantitative reverse … ... development and use of a molecular serotype identification test for infectious bronchitis virus.

Multiplex, DNase-free one-step reverse transcription PCR for ...

Real-time quantitative reverse transcription PCR for monitoring of blood-stage Plasmodium falciparum infections in malaria human challenge ...