- Cross|validation tests for cryo|EM maps using an independent ...🔍
- Single|Particle Cryo|Electron Microscopy🔍
- Super|Resolution Cryo|EM Maps With 3D Deep Generative Networks🔍
- Accelerated cryo|EM structure determination with parallelisation ...🔍
- Machine learning for determining protein structure and dynamics ...🔍
- Novel Developments in Cryo‐EM of Biological Molecules🔍
- algorithms for rapid unsupervised cryo|EM structure determination🔍
- Processing of Structurally Heterogeneous Cryo|EM Data in RELION.🔍
Development of novel 3D classification methods for Cryo
Cross-validation tests for cryo-EM maps using an independent ...
Here, inspired by modern statistics, we develop a novel methodology that uses a small independent particle set to validate the 3D maps. The main idea is to ...
Single-Particle Cryo-Electron Microscopy | Series in Structural Biology
The book reproduces 55 of more than 300 articles written by the author, representing milestones in methods development of single-particle cryo-EM as well as ...
Super-Resolution Cryo-EM Maps With 3D Deep Generative Networks
We report SuperEM, a novel deep learning-based method that uses a three-dimensional generative adversarial network for generating an improved-resolution EM map ...
TomoTwin: generalized 3D localization of macromolecules in cryo ...
Cryogenic-electron tomography (cryo-ET) has emerged as a landmark technique for the visualization of macromolecules within their native cellular ...
(IUCr) Single-particle cryo-EM using alignment by classification (ABC)
A new MSA classification thus yields a better class average since more molecular images now have a common three-dimensional orientation. Shown here are class ...
Accelerated cryo-EM structure determination with parallelisation ...
... development of new methods. ... In practice we believe this will make it more common to use very large numbers of classes in 3D classification.
Machine learning for determining protein structure and dynamics ...
... 3D reconstruction from the collected dataset of 2D cryo-EM images. ... I will discuss various extensions of the method for broadening the scope of ...
Novel Developments in Cryo‐EM of Biological Molecules
... methods of cryo-EM. TABLE OF CONTENTS. part I|74 pages. Single-Particle Cryo-EM of Molecules in Thermal Equilibrium. chapter Chapter 1|15 pages.
algorithms for rapid unsupervised cryo-EM structure determination
However, processing cryo-EM image data to reveal heterogeneity in the protein structure and to refine 3D maps to high resolution frequently becomes a severe ...
Processing of Structurally Heterogeneous Cryo-EM Data in RELION.
A novel methodology is presented, cryoSTAR, that harnesses atomic model information as structural regularization to elucidate such heterogeneity in cryo-EM ...
Computational Methods for Single-Particle Cryo-EM - Princeton Math
The second stage involves methods that operate on individual particle images such as 2D classification, 3D reconstruction, and. 3D heterogeneity ...
Cryo-EM Protocols and Methods | Springer Nature Experiments
The output from Warp can be fed into established programs for particle classification and 3D-map refinement. ... novel therapeutic reagent and to rationally ...
C&SP20: Structural plasticity of chaperonins determined by cryo ...
Using a more advanced image processing method known as focused 3D classification,47 we re-analyzed each of the 14 protein subunits in each of the GroEL particle ...
3D Electron Diffraction: The Nanocrystallography Revolution
3D ED is therefore the technique of choice for the analysis of nanocrystalline mixtures, where XRPD interpretation is hampered by the ...
Cryo-EM. Part B, 3-D reconstruction / edited by Grant J. Jensen.
Penczek -- 3D ... methods in cryo-EM / Fred J. Sigworth [and others] -- Classification of structural heterogeneity by maximum-likelihood methods ...
As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and ...
Stage 2 Build. COMPLETE: First full-scale carbon composite tank built using advanced manufacturing methods · Structural and Cryogenic Testing · Archimedes Engine ...
Chapter 5: Image processing and data analysis - Cryo EM 101
Although 2D classification is not strictly a necessary step along the structure determination pipeline, this procedure can provide invaluable insights.
AlphaFold is an artificial intelligence (AI) program developed by DeepMind, a subsidiary of Alphabet, which performs predictions of protein structure.
Cryo-electron tomography - Kendrew Lecture 2023 - YouTube
... methods developments enabling studies performed in situ, i.e. in unperturbed cellular environments. Sensu stricto the term 'structural ...