- T cell culture🔍
- T|Cell Technology🔍
- ExCellerate™ Human T Cell Expansion Media🔍
- Optimized protocols for γδ T cell expansion and lentiviral transduction🔍
- Automating Primary Human T Cell Culture🔍
- IL|7 Enhances the Survival and Maintains the Size of Naive T Cells1🔍
- 126 questions with answers in T|CELL CULTURE🔍
- Optimized gene engineering of murine CAR|T cells reveals the ...🔍
Primary T Cell Culture and Expansion
T cell culture - Fine-tuning your T cell research workflow step by step
Presented By: Dr. Marcello Stein Speaker Biography: Marcello Stein Ph.D. received his doctoral degree in immunology from the University of ...
T-Cell Technology | Clinical Cell and Vaccine Production Facility ...
The core platform technology at the CVPF is the use of artificial antigen presenting cells (aAPCs) for the expansion of T-cells.
ExCellerate™ Human T Cell Expansion Media, Xeno-Free
The medium supports routine culture of human T cell lines or T cell clones and the stimulation of human ... Results may vary due to variations among primary T ...
Optimized protocols for γδ T cell expansion and lentiviral transduction
Lentiviral transduction is the main method for genetic modification of primary cells and can be used in adoptive antigen-specific T cell therapy ...
Automating Primary Human T Cell Culture - Indee Labs
Primary human T cells were expanded via manual culture (n=3) and automated culture (n=3) on the Monomer work cell. T cells showed no differences ...
IL-7 Enhances the Survival and Maintains the Size of Naive T Cells1
T cells removed from these signals and placed in tissue culture atrophied and died in a caspase-independent manner. Atrophy was characterized by smaller cell ...
126 questions with answers in T-CELL CULTURE | Science topic
We normally use 10ng/mL for CD8+T cell expansion. But you have to pay attention because CD8+ t cells need to be activated or stimulated; otherwise, they die ...
Optimized gene engineering of murine CAR-T cells reveals the ...
Here, we describe optimized methods for the efficient retroviral transduction and expansion of murine T lymphocytes of a predominantly central ...
Culturing T-cells efficiently and effectively - News-Medical
As a key immune cell, T cells have a central role in the development of immune cell therapy culture. With a wide range of T cell culture-related ...
Expansion of Gamma Delta T Cells - A Short Review on ...
In these cytokine-based approaches, the length of culture was often kept between 10 – 14 days (Table 1). Vγ9Vδ2 T cells harvested from day 12 to ...
Chimeric antigen receptor T (CAR-T) cells expanded with IL-7/IL-15 ...
Proliferation and apoptosis are two major aspects to be considered for in vitro expansion of CAR-T cells. In the two-week in vitro culture ...
CAR T Cells: Engineering Immune Cells to Treat Cancer - NCI
Research on CAR T-cell therapy is progressing rapidly to improve the cancer treatment, expand its use to more cancers, and better manage its ...
Successful generation of primary virus-specific and anti-tumor T-cell ...
Antigen-specific CD8 T cells were generated by co-culture of CD45RO-depleted, regulatory T cell-depleted donor peripheral blood mononuclear cells with ...
Activation-induced expression of CD137 permits detection, isolation ...
(B) Enrichment of an HCV/NS3(1406-1415)-specific T-cell line by selection of CD137+ cells. Eight days after primary stimulation of naive T cells, an aliquot was ...
more room for continued growth. Cell Line. After the first subculture, the primary culture becomes known as a cell line or subclone. Cell lines derived from ...
Taken together, these findings demonstrate that the intracel- lular redox environment in T cells changes during culture but is maintained closer ...
Expansion of Autoreactive T Cells in Multiple Sclerosis Is ...
Highly purified CD4 T cells isolated directly ex vivo from peripheral blood were stimulated in primary cultures with self-Ag/MHC complex in the presence or ...
Optimized DNA electroporation for primary human T cell engineering
T cells should be activated before viral transduction, and longer culture periods are required for the integration of exogenous genes into the ...
T cell culture fine-tuned to your cells' needs [WEBINAR] - YouTube
... cell-type/t-cells.html Cell culture is key for efficient and reliable T cell workflows, be it activation and expansion, T helper cell ...
Select sequencing of clonally expanded CD8+ T cells reveals limits ...
Upon binding of the TCR to an antigen–MHC complex, T cells clonally expand to establish an immune response. To study antigen-specific T cell ...