Protein unfolding mechanisms and their effects on folding experiments

The Plastic Energy Landscape of Protein Folding

The results also reconcile the folding mechanisms of top- ological ... The unfolding and refolding kinetic experiments were con- ducted in 50 mM ...

Comparative analysis of folding and unfolding dynamics and free ...

Our results indicate similar force-dependent folding rates for these three proteins, while the unfolding rate of BsCSP, BcCSP, and TmCSP ...

Real-Time Protein Unfolding: A Method for Determining the Kinetics ...

Determining the kinetics of native protein denaturation could provide the researcher with detailed information regarding proteinstructure and the influence of ...

Mechanism of reductive protein unfolding - Nature

The reductive unfolding of ribonuclease A with dithiothreitol proceeds through parallel pathways with the formation of two well-populated ...

Exploration of Protein Unfolding by Modelling Calorimetry Data from ...

experimental folding/unfolding data and in silico protein models and ... the main motivation for understanding the unfolding mechanism of proteins ...

Fast protein folding kinetics | Quarterly Reviews of Biophysics

This coupled study of fast-folding proteins has provided insight into the mechanisms, which allow some proteins to find their native conformation well <1 ms and ...

The Road Less Traveled in Protein Folding: Evidence for Multiple ...

Another limitation of single molecule experiments is the need to work under conditions in which multiple folding and unfolding events can be reported for ...

Characterization of the Protein Unfolding Processes Induced by ...

characterize the effect of pressure and temperature on the molecular mechanisms of protein unfolding. ... Sampling the multiple folding mechanisms of Trp ...

The Search for Folding Intermediates and the Mechanism of Protein ...

Using hydrogen exchange as a probe, we found the rapid formation of secondary structure during folding and used this to provide an NMR pulse ...

Interaction Networks in Protein Folding via Atomic-Resolution ...

Here, we combine equilibrium NMR measurements of thermal unfolding and long molecular dynamics simulations to investigate the folding of gpW, a ...

Funnels, pathways, and the energy landscape of protein folding

The understanding, and even the description of protein folding is impeded by the complexity of the process. Much of this complexity can be described and ...

Understanding protein folding via free-energy surfaces from theory ...

Analysis of these surfaces, derived from the constructive interplay between theory and experiment, has led to the development of a unified mechanism for folding ...

Protein Unfolding: Denaturant vs. Force - MDPI

It is these differences that established the hypothesis that refolding might be initiated at different points on the folding funnel based on the method of ...

Protein Folding: Basics, Driving Forces, Experimental ... - YouTube

Protein folding is the process by which a linear chain of amino acids folds into its three-dimensional structure. The folded structure is ...

Kinetic folding mechanism of PDZ2 from PTP-BL - Oxford Academic

The complex dependence of the unfolding rate constants on denaturant concentration allows us to estimate the position in the folding reaction coordinate of two ...

New insights into the folding–unfolding mechanism and ...

Metalloproteins account for over one-third of all proteins in nature and play important roles in biological processes. The formation of the ...

Unified understanding of folding and binding mechanisms of ...

Multi-subdomain proteins fold mainly by the induced-fit (hydrophobic collapse) mechanism, as the connection of interacting segments enhances the ...

Protein Folding Under Mechanical Forces: A Physiological View

Perturbation method affects the unfolded pro- tein conformation and thus folding mechanism as such; proteins that unfold with chemical denatur- ants appear ...

Microscopy study of protein folding & unfolding mechanisms

Why? Because a knowledge of a protein's structural dynamics is central to the understanding of many neurological diseases ranging from brain tumors to ...

Protein folding and misfolding: mechanism and principles

Proteins act like accretions of cooperative unfolding/refolding units called foldons which account for the unit steps in folding pathways. The foldon ...