Events2Join

Confocal Versus Super|resolution Microscopy


How is the resolution of a Laser Scanning Microscope defined?

The focused excitation laser makes a certain volume to become fluorescent. The size of this confocal volume in μm2 depends on the excitation ...

Super-Resolution Microscopy Accessible to All - Andor Technology

Or does not have a spinning disk confocal microscope? Is there an alternative method that is fast, compatible with live imaging, and that can still acquire ...

Super-resolution microscopy of mitochondria - ScienceDirect.com

Although these methods improve the resolution compared to confocal microscopy, they do not allow substantially better resolution than ∼100 nm.

Super-Resolution Microscopy On Standard Confocal Microscopes

The Nobel Prize-winning innovation of super-resolution microscopy, which allows imaging below the diffraction limit of light, ...

Light Sheet vs. Confocal Microscopy for 3D Imaging

Laser scanning confocal microscopy is a powerful imaging technique that is particularly well-suited for capturing high-resolution images of smaller samples.

Confocal microscopy - Wikipedia

Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM) or laser scanning confocal microscopy (LSCM), is an optical imaging technique ...

Super-Resolution Microscopes | Bruker

Super-resolution microscopy techniques, such as single molecule localization microscopy (SMLM), have been created to overcome some of the key challenges ...

Microscopes – Optical Imaging Core – UW–Madison

Leica SP8 3X STED Confocal/Super-Resolution Microscope $30/hour ($90/hr for ... The W1 provides a wider field of view as compared to previous spinning disk ...

Super‐Resolution Confocal Microscopy Through Pixel Reassignment

Additional information is available at the end of the chapter Abstract Confocal microscopy has gained great popularity in the observation of ...

The cell biologist's guide to super-resolution microscopy

STED microscopy allows the user to directly visualize the object of interest in super resolution without the need for offline computation or ...

Super-resolution Microscopy

1. Different super-resolution technologies are available, but they are generally built around conven- tional confocal or widefield fluorescence microscopes.

Super-resolution confocal microscopy enhanced by deep-learning

Precise over-sampling of an object can produce super-resolution images, as demonstrated in the well-known multi-frame super-resolution scheme. The application ...

Confocal Microscopy - Edmund Optics

Confocal microscopy is a type of fluorescence microscopy which uses a laser to excite fluorescence from fluorophores used to label different subsets of a ...

Ultrafast superresolution fluorescence imaging with spinning disk ...

On the basis of this theory, we modified a commercial spinning disk confocal microscope. The improved resolution around 120 nm was confirmed ...

Confocal Microscopy | Principle & Applications - ibidi

Similar to the widefield microscope, the confocal microscope uses fluorescence optics. Instead of illuminating the whole sample at once, laser light is focused ...

Super resolution microscopy - Possibility | Teledyne Imaging

Super resolution techniques use a variety of methods to break the diffraction limit of light, and can be broken down into a few main categories.

The Differences Between Widefield and Confocal Microscopy

Widefield microscopy is fast and easy to use, while confocal microscopy provides higher resolution and 3D imaging capabilities.

Superresolution microscopy - UHasselt

advantages, disadvantages ; very versatile (balance speed, resolution and SNR), "only" up to a twofold resolution improvement ; no drawbacks compared to confocal ...

GAIA | Point-Rescanning Confocal Super-Resolution Microscopy

In laser rescanning confocal microscopy, the magnification of the image and the spot are decoupled. Thus, one can magnify the image compared to the spot. In RCM ...

Computational methods in super-resolution microscopy

Confocal fluorescence microscopy is popular among biologists for its superior sectioning ability. In confocal microscopy, a laser is usually ...