[PDF] A Mechanistic Model of PCR for Accurate Quantification of ...

A Mechanistic Model of PCR for Accurate Quantification of ...

In theory, the shape of a qPCR data curve can be used to directly quantify DNA concentration by fitting a model to data; however, current ...

(PDF) Boggy, G.J. & Woolf, P.J. A mechanistic model of PCR for ...

Our results indicate that quantification by MAK2-fitting is as reliable as standard curve quantification for a variety of DNA targets and a wide range of ...

[PDF] A Mechanistic Model of PCR for Accurate Quantification of ...

A two-parameter mass action kinetic model of PCR (MAK2) that can be fitted to qPCR data in order to quantify target concentration from a singleqPCR assay is ...

A Mechanistic Model of PCR for Accurate Quantification of ...

Quantitative PCR (qPCR) is a workhorse laboratory technique for measuring the concentration of a target DNA sequence with high accuracy over a wide dynamic ...

(PDF) A mechanistic model of PCR for accurate quantification of ...

TL;DR: A two-parameter mass action kinetic model of PCR (MAK2) that can be fitted to qPCR data in order to quantify target concentration from a singleqPCR ...

A mechanistic model of PCR for accurate quantification of ... - PubMed

We anticipate that MAK2 quantification will have a profound effect on the way qPCR experiments are designed and analyzed.

A Mechanistic Model of PCR for Accurate Quantification of ...

Published in PLoS ONE by Public Library of Science (PLoS). 2010 e12355. Archived Files and Locations. application/pdf 371.1 kB file_ybsz5avpuzdpvd26papqwdof34 ...

A Mechanistic Model of PCR for Accurate Quantification of ... - PLOS

Figure 1. Simulated MAK2 curves with varying and values. Curves are labeled with parameter values. Increasing shifts the MAK2 curve to the left, while ...

a reliable and accurate approach for qPCR analysis | BMC ...

Download PDF. Research; Open access; Published: 11 ... A mechanistic model of PCR for accurate quantification of quantitative PCR data.

qPCR data analysis: Better results through iconoclasm - ScienceDirect

... PDF file (1MB). Recommended articles. References. [1]. D. Svec, A. Tichopad ... A mechanistic model of PCR for accurate quantification of quantitative PCR data.

Mathematical model of real-time PCR kinetics. - Semantic Scholar

Expand. 75 Citations · PDF. Add to Library. Alert. A Mechanistic Model of PCR for Accurate Quantification of Quantitative PCR Data · Gregory J BoggyP. Woolf.

Comparing real-time quantitative polymerase chain reaction ...

Boggy, P.J. Woolf, A mechanistic model of PCR for accurate quantification · of quantitative PCR data, PLoS ONE 5 (2010) e12355. [48] D. Sisti, M. Guescini ...

A survey of tools for the analysis of quantitative PCR (qPCR) data

... PDF, SVG, HTML, and XLS). Tool, Web, Feature, Cq ... Boggy G.J., Woolf P.J. A mechanistic model of PCR for accurate quantification of quantitative PCR data.

Counting PCR: A new method to obtain absolute DNA copy number ...

Boggy, G. J. & Woolf, P.J. (2010). “A mechanistic model of PCR for accurate quantification of quantitative PCR data.” PLoS ONE5(8): 355.

A Mechanistic Model of PCR for Accurate Quantification of ... - OUCI

A Mechanistic Model of PCR for Accurate Quantification of Quantitative PCR Data · List of references · Publications that cite this publication.

Robust and Precise Quantitative Real-Time Polymerase Chain ...

Robust and Precise Quantitative Real-Time Polymerase Chain Reaction with an Amplification Efficiency-Aware Reaction Kinetics Model ... To read the ...

Robust Quantification of Polymerase Chain Reactions Using Global ...

Unlike other PCR models, this equation enables accurate modeling of entire data sets and is unaffected by cycle number, curve shape, or plateau ...

arXiv:2103.00408v1 [q-bio.QM] 28 Feb 2021

indicate that the CT method is more precise for absolute quantification than 11 mechanistic models [15]. More im- portantly, fitting of ...

System-specific periodicity in quantitative real-time polymerase ...

Boggy, G. J. & Woolf, P. J. A mechanistic model of PCR for accurate quantification of quantitative PCR data. ... pdf/lsr/literature/Bulletin_6047A ...

Evaluation validation of a qPCR curve analysis method and ...

Download PDF. Volume 22 Supplement 5. Selected articles ... A mechanistic model of PCR for accurate quantification of quantitative PCR data.