16S Ribosomal RNA PCR Versus Conventional Diagnostic Culture ...

The purpose of this study was to compare conventional diagnostic culture (CDC) to 16S ribosomal RNA polymerase chain reaction (PCR) analysis for diagnosing ...

Broad-Range 16S rRNA Gene Polymerase Chain Reaction for ...

Results from 394 specimens showed a high concordance of >90% for 16S rRNA gene PCR and routine bacterial culture, indicating that the diagnostic performance of ...

Purpose: The purpose of this study was to compare conventional diagnostic culture. (CDC) to 16S ribosomal RNA polymerase chain reaction (PCR) ...

(PDF) 16S Ribosomal RNA PCR Versus Conventional Diagnostic ...

In CDC negative samples, 16S PCR identified more pathogens than CDC in 16S PCR negative samples. Neither order of sample collection nor prior ...

16S Ribosomal RNA PCR Versus Conventional Diagnostic Culture ...

Erratum in: 16S Ribosomal RNA PCR Versus Conventional Diagnostic Culture in the Investigation of Suspected Bacterial Keratitis.

Clinical utility and cost-effectiveness of bacterial 16S rRNA ... - Nature

16S ribosomal-ribonucleic acid polymerase chain reaction (PCR) and targeted PCR aid microbiological diagnosis in culture-negative clinical ...

Erratum in: 16S Ribosomal RNA PCR Versus Conventional ...

Erratum in: “16S Ribosomal RNA PCR Versus Conventional Diagnostic Culture in the Investigation of Suspected Bacterial Keratitis” by Tobi F. Somerville, ...

Comparison of 16S Ribosomal RNA Targeted Sequencing and ...

16S rRNA-targeted sequencing in conjunction with culture could be useful for identifying bacteria in NSBF samples, especially when patients have ...

Microbiological culture versus 16S/18S rRNA gene PCR-sanger ...

Despite having the lowest but comparable sensitivity, 16S rRNA PCR was able to improve the diagnostic yield in direct culture-negative cases by ~10%, ...

16S Ribosomal RNA PCR Versus Conventional Diagnostic Culture ...

16S rRNA gene PCR performed in addition to CDC on corneal samples from patients with clinically suspected bacterial keratitis led to additional pathogen ...

16S Ribosomal RNA PCR Versus Conventional Diagnostic Culture ...

Using a combination of both PCR and CDC increased the detection rate for pathogenic bacteria by 13% compared to using CDC alone (P = 0.04). In CDC negative ...

Comparison between a Broad-Range Real-Time and a Broad ...

It constitutes a reliable and complementary diagnostic tool to the bacterial culture. Keywords. 16S rRNA · real-time PCR · broad-range PCR · molecular diagnosis.

16S rRNA Sequencing: A PCR-based Technique to Identify ... - JoVE

A popular method is 16S ribosomal RNA (rRNA) gene sequencing. This method is not only faster and more accurate than conventional methods, but also allows ...

Results for samples referred for 16s PCR versus conventional culture...

Using the final diagnosis as a gold standard, the sensitivity of 16S rRNA gene sequencing was fair. In the setting with no 16S rRNA gene sequencing test ...

Utility of 16S rRNA PCR performed on clinical specimens in patient ...

Despite the low diagnostic yield, results of 16S rRNA PCR can still have a significant impact on patient management due to rationalization or cessation of the ...

Evaluation of 16S rRNA Gene PCR Sensitivity and Specificity for ...

Broad-range 16S rRNA gene PCR analysis has already been evaluated for the diagnosis of PJI; 16S rRNA gene PCR assays of periprosthetic tissue or periprosthetic ...

16S Ribosomal RNA PCR Versus Conventional Diagnostic Culture ...

16S Ribosomal RNA PCR Versus Conventional Diagnostic Culture in the Investigation of Suspected Bacterial Keratitis · List of references · Publications that cite ...

Microbiological culture versus 16S/18S rRNA gene PCR ... - Frontiers

Background: To compare the diagnostic performance of microbiological culture and 16S/18S rRNA gene polymerase chain reaction (PCR)-Sanger ...

Evaluation of 16S rRNA broad range PCR assay for microbial ...

Broad range PCR assay was far superior for early diagnosis of infection. The bacteria which could not be detected by culture and were not commonly reported from ...

Diagnosis of Bacteremia in Whole-Blood Samples by Use of a ...

PCR amplification of conserved regions of the bacterial genome, in particular the 16S rRNA gene, combined with sequence analysis is a well-established technique ...