A CRISPR/dCas9‐enabled

CRISPR/dCas9-Based Systems: Mechanisms and Applications in ...

dCas12a can process a single transcript tandem crRNA array to multiple crRNAs on its own, enabling a more simplified multiplex transcriptional repression ...

A CRISPR/dCas9‐enabled, on‐site, visual, and bimodal biosensing ...

Abstract. It is imperative to develop practicable pathogenic bacteria detection methods. We devised a biosensor for the ultrasensitive detection ...

CRISPR/dCas9 Tools: Epigenetic Mechanism and Application in ...

The dCas9p300-core (WT) fusion protein promoted histone acetylation of the target gene promoter and distal regulatory element and activated ...

CRISPR-based Gene Activation - Sigma-Aldrich

The dCas9-p300 CRISPR Gene Activator system is based on a fusion of dCas9 to the catalytic histone acetyltransferase (HAT) core domain of the human E1A- ...

Generation of a CRISPR activation mouse that enables modelling of ...

Generation of CRISPRa transgenic mice. For flexible expression of the CRISPRa system in vivo, we targeted the dCas9-VP64-T2A-MS2-p65-HSF1 ...

A digital CRISPR-dCas9-based gene remodeling biocomputer ...

Moreover, REPACRISPRa enables logic operations on multiplexed endogenous gene transcription. We have also established five mathematical models ...

A CRISPR/dCas9-based transcription activated system developed in ...

A transcriptional activated system based on CRISPR/dCas9 constructed. CRISPRa efficiently works in Nannochlorpsis. Transcript of target gene increased by ...

dCas9-based gene editing for cleavage-free genomic knock-in of ...

Overall, the dCas9–SSAP editor is capable of efficient, accurate knock-in genome editing. With space for further improvement, it is a valuable ...

On-target activity predictions enable improved CRISPR–dCas9 ...

We fit a model to predict the ability of dCas9 to block the RNA polymerase based on the target sequence, and validate its performance on independently ...

A Neuron-Optimized CRISPR/dCas9 Activation System for Robust ...

We report a neuron-optimized CRISPR/dCas9 activation (CRISPRa) system that produces robust and specific upregulation of targeted genes in neurons both in vitro ...

CRISPR activation - Wikipedia

CRISPR activation (CRISPRa) is a gene regulation technique that utilizes an engineered form of the CRISPR-Cas9 system to enhance the expression of specific ...

CRISPR activation - Horizon Discovery

This dCas9 protein is further engineered by fusing various transcriptional activators. The Dharmacon CRISPRmod CRISPRa system relies on the popular dCas9-VPR ...

Ezh2-dCas9 and KRAB-dCas9 enable engineering of epigenetic ...

Here, we report that persistent epigenetic memory required both a DNA methyltransferase (DNMT3A-dCas9) and a histone methyltransferase (Ezh2-dCas9 or KRAB-dCas ...

Tuning dCas9's ability to block transcription enables robust ...

The catalytically dead variant of Cas9 known as dCas9 can be guided by small RNAs to block transcription of target genes, in a strategy also known as CRISPRi.

Quantitative and modularized CRISPR/dCas9-dCpf1 dual function ...

Additionally, the gRNA-protein complex enables the design of multiple gRNAs to accurately regulate targeted DNA regions. However, it is worth ...

Synthetic far-red light-mediated CRISPR-dCas9 device for inducing ...

The ability to control the activity of CRISPR-dCas9 with precise spatiotemporal resolution will enable tight genome regulation of ...

4) CRISPR Cas9 - Gene Regulation with dCas9 - YouTube

... crispr-cas9-gene-regulation-dCas9 Want more tips, tricks, guides, and protocols on how to knockout a gene using CRISPR? Join our free, 4 ...

A Cre-Dependent CRISPR/dCas9 System for Gene Expression ...

While CRISPR/dCas9 approaches have enabled targeted induction of transcriptional or epigenetic states at selected genes, inducible cell type ...

CRISPR/dCas9 platforms in plants: strategies and applications ...

Originally, dCas9 was used as a CRISPR/Cas9 re-engineering tool that enables targeted expression of any gene or multiple genes through ...

Questions and Answers about CRISPR | Broad Institute

A: CRISPR-Cas9 is proving to be an efficient and customizable alternative to other existing genome editing tools. Since the CRISPR-Cas9 system itself is capable ...