A Case Study with Mycobacterium DNA Binding Protein

We have shown that, when a kinetic bottleneck is applied, the binding mechanism showed cooperative binding (n = 2.72) at lower temperatures.

Mycobacterial DNA-binding protein 1 is critical for BCG survival in ...

Contrary to our findings, a previous study reported that MDP1 suppression led to accelerated BCG growth in vitro as quantified by ATP levels.

The mycobacterial DNA-binding protein 1 (MDP1 ... - PubMed Central

Primers and probe used in this study. Gene, Primer pair, probe, Sequences of primers and probesa, References accession no. MDP1 ...

(PDF) Nonspecific Interaction between DNA and Protein allows for ...

ArticlePDF Available. Nonspecific Interaction between DNA and Protein allows for Cooperativity: A Case Study with Mycobacterium DNA Binding Protein. November ...

Recombinant mycobacterial DNA-binding protein 1 with post ...

Tuberculosis remains a large health threat, despite the availability of the tuberculosis vaccine, BCG. As BCG efficacy gradually decreases ...

Structural basis of DNA binding by the WhiB-like transcription factor ...

Other Mtb Wbl proteins except WhiB5 have also been reported to bind to σA4 in a [4Fe-4S]-dependent manner (23). Moreover, a recent study has shown that the ...

Limited proteolysis of mycobacterial DNA-binding protein 1 with an ...

In the present study, we implicated varying short times of digestion with trypsin (limited proteolysis) in proteomic analysis to generate a significant number ...

Mycobacterium tuberculosis Ku C-terminus is a multi-purpose arm ...

Bacterial NHEJ is a minimalist version of eukaryotic NHEJ, consisting primarily of a Ku protein for binding DNA and recruiting the ATP-dependent ...

DNA structural properties of DNA binding sites for 21 transcription ...

Mycobacterium tuberculosis, the causative agent of tuberculosis, has evolved over time into a multidrug resistance strain that poses a ...

DNA Augments Antigenicity of Mycobacterial DNA-Binding Protein 1 ...

In this study we report a series of studies that analyze the antigenicity of MDP1 in a mouse model. We show that both humoral and cellular immune responses to ...

Mechanism of DNA organization by Mycobacterium tuberculosis ...

Here, we report that Lsr2 cooperatively binds to DNA forming a rigid Lsr2 nucleoprotein complex that restricts DNA accessibility, similar to H-NS.

Nucleoid‐associated proteins of mycobacteria come with a ...

Binding studies using truncated forms of M. smegmatis HU revealed that the two domains can bind DNA independently in a nonspecific manner, and ...

Identification of the mycobacterial DNA-binding protein 1 region ...

In this study, we examined the DNA binding regions of MDP1 by using a set of synthetic peptides. One dominant region was determined on peptide 4 composed of ...

The Mycobacterium bovis BCG Cyclic AMP Receptor-Like Protein Is ...

The Mycobacterium bovis BCG Cyclic AMP Receptor-Like Protein Is a Functional DNA Binding ... We report here that CRPBCG is a fully functional DNA binding protein ...

A Genetic Analysis of the Functional Interactions within ... - PLOS

Single-stranded DNA binding proteins (SSBs) are vital in all organisms. SSBs of Escherichia coli (EcoSSB) and Mycobacterium tuberculosis (MtuSSB) are ...

Mycobacterium tuberculosis WhiB1 is an essential DNA-binding ...

tuberculosis has seven such proteins. In the present study it is shown that the M. tuberculosis H37Rv whiB1 gene is essential. The WhiB1 protein possesses a [ ...

Structural analysis reveals DNA binding properties of Rv2827c, a ...

Tuberculosis (TB) is a major global health threat caused by Mycobacterium tuberculosis (Mtb). It is further fueled by the HIV pandemic and ...

Characterization and Function in Escherichia coli | PLOS ONE

Single stranded DNA binding proteins (SSBs) are vital for the survival of organisms. Studies on SSBs from the prototype, Escherichia coli (EcoSSB) and, ...

altered DNA binding and gene regulation - Wiley Online Library

HU and Lsr2, two nucleoid-associated proteins of Mycobacterium tuberculosis form a complex having DNA architectural and regulatory pattern ...

The DNA-binding network of Mycobacterium tuberculosis. - Abstract

These proteins may regulate other TFs and initiate a transcriptional cascade. Alternatively, some of these TFs may be poor candidates for ChIP analysis. In ...