A Mechanistic Model of PCR for Accurate Quantification of ...

A Mechanistic Model of PCR for Accurate Quantification of ... - PLOS

We have developed a two-parameter mass action kinetic model of PCR (MAK2) that can be fitted to qPCR data in order to quantify target concentration from a ...

A mechanistic model of PCR for accurate quantification of ... - PubMed

We anticipate that MAK2 quantification will have a profound effect on the way qPCR experiments are designed and analyzed.

[PDF] A Mechanistic Model of PCR for Accurate Quantification of ...

A two-parameter mass action kinetic model of PCR (MAK2) that can be fitted to qPCR data in order to quantify target concentration from a singleqPCR assay is ...

(PDF) Boggy, G.J. & Woolf, P.J. A mechanistic model of PCR for ...

Quantitative PCR (qPCR) is a workhorse laboratory technique for measuring the concentration of a target DNA sequence with high accuracy over a wide dynamic ...

A Mechanistic Model of PCR for Accurate Quantification of ... - PLOS

Each dataset is from a different target sequence diluted sequentially by ten-fold to obtain data from a concentration range of six orders of magnitude. Panels ...

A Mechanistic Model of PCR for Accurate Quantification of ...

A Mechanistic Model of PCR for Accurate Quantification of Quantitative PCR Data. Boggy, Gregory J. ;; Woolf, Peter J. Abstract. Publication: PLoS ONE. Pub Date ...

A Mechanistic Model of PCR for Accurate Quantification of ... - OUCI

A Mechanistic Model of PCR for Accurate Quantification of Quantitative PCR Data · List of references · Publications that cite this publication.

(PDF) A mechanistic model of PCR for accurate quantification of ...

TL;DR: A two-parameter mass action kinetic model of PCR (MAK2) that can be fitted to qPCR data in order to quantify target concentration from a singleqPCR ...

A Mechanistic Model of PCR for Accurate Quantification ... - Altmetric

A Mechanistic Model of PCR for Accurate Quantification of Quantitative PCR Data · PLOS ONE, August 2010 · 10.1371/journal.pone.0012355 · 20814578 · Gregory J. Boggy ...

A Mechanistic Model of PCR for Accurate Quantification of ... - Paperity

Background Quantitative PCR (qPCR) is a workhorse laboratory technique for measuring the concentration of a target DNA sequence with high accuracy over a ...

A Mechanistic Model of PCR for Accurate Quantification of ...

A Mechanistic Model of PCR for Accurate Quantification of Quantitative PCR Data release_vo3hncetcrgy7asrgth4seftvi. by Gregory J. Boggy, Peter J. Woolf.

a reliable and accurate approach for qPCR analysis | BMC ...

Quantitative polymerase chain reaction (qPCR) allows the quantification of minute amounts of a specific target DNA by monitoring the increase in ...

Evaluation validation of a qPCR curve analysis method and ... - NCBI

Reverse Transcription quantitative polymerase chain reaction (RT-qPCR) is a sensitive and reliable method for mRNA quantification and rapid ...

System-specific periodicity in quantitative real-time polymerase ...

Real-time quantitative polymerase chain reaction (qPCR) data are found to display periodic patterns in the fluorescence intensity as a function of sample ...

Determination of PCR Efficiency (main) - Gene-Quantification

One has to consider two key factors in precise statistical analysis of real-time PCR data: a well-defined statistical model and the integration ...

qPCR data analysis: Better results through iconoclasm - ScienceDirect

A new mathematical model for relative quantification in real-time RT-PCR ... A mechanistic model of PCR for accurate quantification of quantitative PCR data.

The nonlinear/mechanistic models implemented in qpcR - rdrr.io

Standardized determination of real-time PCR efficiency from a single reaction set-up. Tichopad A, Dilger M, Schwarz G & Pfaffl MW. Nucleic Acids Res (2003), 31: ...

Mathematical model of real-time PCR kinetics. - Semantic Scholar

A mathematical model of the annealing and extension phases of the PCR process is developed that allows us to predict the efficiency of rtPCR at any cycle ...

Real-time polymerase chain reaction - Wikipedia

It monitors the amplification of a targeted DNA molecule during the PCR (i.e., in real time), not at its end, as in conventional PCR. Real-time PCR can be used ...

A survey of tools for the analysis of quantitative PCR (qPCR) data

Real-time quantitative polymerase-chain-reaction (qPCR) is a standard technique in most laboratories used for various applications in basic research.