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A new pattern in antibody light chain tetramer formation


A new pattern in antibody light chain tetramer formation

The state where four antibody light chains aggregate to form a tetramer is in equilibrium with the monomeric state.

Structural and thermodynamic insights into antibody light chain ...

Overexpression of antibody light chains in small plasma cell clones can lead to misfolding and aggregation. On the other hand, the formation ...

The structure of a typical antibody molecule - Immunobiology - NCBI

Each arm of this Y-shaped structure is formed by the association of a light chain with the amino-terminal half of a heavy chain, whereas the trunk of the Y is ...

Structural and Thermodynamic Insights into Antibody Light Chain ...

Here, a pair of domain-swapped dimers creates a tetramer through hydrophobic interactions, facilitating the revelation of the domain-swapped ...

Novel insights into antibody aggregation expected to open up new ...

However, their light chains—parts of the antibody that contribute to recognizing and binding to specific antigens—misfold and aggregate, leading ...

Structure, dynamics and transferability of the metal-dependent ...

Here, we obtained a 2.5 Å resolution crystal structure of a single chain Fv antibody (scFv) bearing a C-terminal sortase motif, 6XHis and ...

Immunoglobulin Light Chain - an overview | ScienceDirect Topics

Immunoglobulin light chain (IgLC) is a component of antibodies, but its free form is observed in the circulation, which originates from 10 to 40% excess ...

Antibodies gone bad – the molecular mechanism of light chain ...

Fibrils also exhibit a characteristic X-ray diffraction pattern [[86-88]] as a defining feature. Detailed analyses of different amyloid fibrils ...

Structural and thermodynamic insights into antibody light chain ...

Here, a pair of domain-swapped dimers creates a tetramer through hydrophobic interactions, facilitating the revelation of the domain-swapped structure. The ...

Ex vivo characterization and isolation of rare memory B cells with ...

To conclusively demonstrate the specificity of TTCF tetramer staining, we generated 5 fully human recombinant antibodies that were randomly chosen from 2 ...

Antibody Structure and Function: The Basis for Engineering ... - MDPI

The pairing of VL and VH, form the antigen-binding site. The two β-sheets formed with β-strands ↓C'' ↑C' ↓C ↑F ↓B pack together, forming a barrel- ...

CDR loop interactions can determine heavy and light chain pairing ...

Typically antibodies consist of two identical heavy and light chains and are characterized by a unique modular anatomy that facilitates their engineering and ...

Aggregation mechanism and branched 3D morphologies of ...

Aggregation of human myeloma immunoglobulin light chain (hLC) upon thiol reduction. ... TCEP, DTT and GSH induced thiol reduction display different aggregation ...

Polymerization of Immunoglobulin Domains - OSTI.GOV

This property, as exhibited by immunoglobulin light chains (normally a component of an intact antibody molecule), may have novel implications for the.

What is new in diagnosis and management of light chain amyloidosis?

Inhibition by small-molecule ligands of formation of amyloid fibrils of an immunoglobulin light chain variable domain [published online ahead of print 18 ...

Inhibition by small-molecule ligands of formation of amyloid fibrils of ...

Overproduction of immunoglobulin light chains leads to systemic amyloidosis, a lethal disease characterized by the formation of amyloid ...

First observation of structures resulting from 3D domain swapping in ...

The 3D-DS of the antibody light chain involves the formation of dimers (structures consisting of two identical subunits) and tetramers ( ...

Fatal amyloid formation in a patient's antibody light chain is caused ...

In systemic light chain amyloidosis, an overexpressed antibody light chain (LC) forms fibrils which deposit in organs and cause their ...

Molecular Mechanism of Antibody-Mediated Activation of &beta

et al., 1978), thus stimulating tetramer formation. In Figure 4, we ... within the Fv structure corresponded to the light chain and heavy chain sequences.

Assembly of Immunoglobulin Light Chains as a Prerequisite for ...

We show that the export-incompetent mutant λ1 FS62 chain forms disulfide bonds in both domains only upon pairing with Ig H chain and is secreted as part of an ...