Accurate and sensitive quantification of protein|DNA binding affinity

Accurate and sensitive quantification of protein-DNA binding affinity

This study presents the first computational method able to quantify the sequence-affinity relationship almost perfectly over the full affinity range.

Accurate and sensitive quantification of protein-DNA binding affinity

Transcription factors (TFs) control gene expression by binding to genomic DNA in a sequence-specific manner. Mutations in TF binding sites ...

Accurate and sensitive quantification of protein-DNA binding affinity

This study presents the first computational method able to quantify the sequence-affinity relationship almost perfectly over the full affinity range. It ...

Accurate and sensitive quantification of protein-DNA binding affinity

The affinity with which DNA is bound by a particular TF can vary more than a thousand-fold with different DNA sequences. This study presents the ...

DNA–protein interaction studies: a historical and comparative analysis

It is a very sensitive method that directly measures binding affinities and kinetics simultaneously. Furthermore, this technique does not ...

Accurate and sensitive quantification of protein-DNA binding affinity

Chaitanya Rastogi speaking at BIRS workshop, Rules of Protein-DNA Recognition: Computational and Experimental Advances, on Tuesday, June 5, ...

Protein–DNA/RNA Interactions: An Overview of Investigation ...

The unbiased procedure defined as affinity purification–mass spectrometry (AP-MS) is based on the combination of affinity isolation strategies with MS ...

Accurate and Sensitive Quantification of Protein-DNA Binding Affinity

Our results establish a powerful paradigm for identifying protein binding sites and interpreting gene regulatory sequences in eukaryotic genomes. Full Text.

Determining DNA–Protein Binding Affinities and Specificities from ...

Apparent dissociation constants (KdApps), a measure of binding affinity, can be calculated by determining the DNA concentration at which 50% of ...

Prediction of protein–ligand binding affinity from sequencing data ...

Here we describe a flexible machine learning method, called ProBound, that accurately defines sequence recognition in terms of equilibrium binding constants or ...

Methods for Detecting Protein-DNA Interactions - US

detect low abundance DNA binding proteins from lysates · test binding site mutations using many probe configurations with the same lysate · test binding affinity ...

Quantifying DNA–protein binding specificities by using ... - PNAS

Here, we develop a in vitro method for sensitive, multiplex quantification of DNA–protein binding specificities by using oligonucleotide mass tags (OMTs) and ...

Accurate and sensitive quantification of protein-DNA binding affinity

Accurate and sensitive quantification of protein-DNA binding affinity · About this Attention Score · Mentioned by · Citations · Readers on.

PDA-Pred: Predicting the binding affinity of protein-DNA complexes ...

Rastogi et al. [10] proposed a method to quantify the sequence-affinity relationship by analyzing data from DNA sequencing experiments to comprehensively probe ...

Is there a technique for quantification of the binding affinity ... - Quora

The accuracy of your procedure depends on the half-life of your protein. If it takes 5 minutes to bind and elute the protein from your affinity ...

DNA-protein binding by fluorescence anisotropy | BMG LABTECH

Total ionic strength was 286 mM. Bovine serum albumin (BSA, 0.2 mg/mL) was added to buffers to minimise non-specific binding of proteins. The concentration of ...

Transcription Factor Binding Affinities and DNA Shape Readout

Here, we use a binding assay we recently established to measure with high sensitivity the binding specificities of 13 Drosophila TFs, including ...

Geometric deep learning of protein–DNA binding specificity - Nature

Protein–DNA complexes usually exhibit binding to a selected DNA target site, whereas a protein binds, with varying degrees of binding ...

Structural predictions of protein–DNA binding: MELD-DNA

The consensus sequence is the highest affinity binder of all sequences. However, other sequences are also likely to bind based on charge ...

Accurate and Sensitive Quantification of Protein-DNA Binding Affinity

Mutations in transcription factor binding sites are increasingly found to be associated with human disease, yet we currently lack robust methods to predict ...