- Accurate expression quantification from nanopore direct RNA ...🔍
- Enhancing transcriptome expression quantification through accurate ...🔍
- Sequencing accuracy and systematic errors of nanopore direct RNA ...🔍
- Advances in nanopore direct RNA sequencing🔍
- Gene expression🔍
- Pervasive effects of RNA degradation on Nanopore direct RNA ...🔍
- Oxford Nanopore RNA seq enables precise RNA isoform ...🔍
- Nanopore direct RNA sequencing detects differential expression ...🔍
Accurate expression quantification from nanopore direct RNA ...
Accurate expression quantification from nanopore direct RNA ...
We developed NanoCount for fast, accurate transcript isoform quantification in DRS and demonstrate it outperforms similar methods.
Accurate expression quantification from nanopore direct RNA ...
Accurately quantifying gene and isoform expression changes is essential to understanding cell functions, differentiation and disease.
Accurate expression quantification from nanopore direct RNA ...
Abstract Sequencing full-length native RNAs using long-read direct RNA sequencing (DRS) has the potential to overcome many limitations of ...
Accurate expression quantification from nanopore direct RNA ...
Accurate expression quantification from nanopore direct RNA sequencing with NanoCount | Welcome to Oxford Nanopore technologies.
Enhancing transcriptome expression quantification through accurate ...
Specifically, we utilized a dataset provided by the Singapore Nanopore Expression Project (SG-Nex), consisting of ONT direct RNA reads sequenced ...
(PDF) Accurate expression quantification from nanopore direct RNA ...
Genes upregulated in neuron-like cells were associated with neurogenesis. NanoCount quantification of thousands of novel isoforms discovered ...
Sequencing accuracy and systematic errors of nanopore direct RNA ...
Direct RNA sequencing (dRNA-seq) on the Oxford Nanopore Technologies (ONT) platforms can produce reads covering up to full-length gene ...
Advances in nanopore direct RNA sequencing | Nature Methods
Aligned read lengths ranged from 85 to >21,000 nucleotides with a median basecall accuracy of 86%. Fig. 1: Nanopore sequencing of individual E.
Gene expression | Oxford Nanopore Technologies
Accurately characterise and quantify full-length transcripts with nanopore reads of unrestricted length. Explore epigenetic modifications through direct RNA ...
Pervasive effects of RNA degradation on Nanopore direct RNA ...
Oxford Nanopore direct RNA sequencing (DRS) is capable of sequencing complete RNA molecules and accurately measuring gene and isoform expression ...
Oxford Nanopore RNA seq enables precise RNA isoform ... - YouTube
... RNA molecules in a single read, allowing researchers to accurately quantify expression for the complete set of RNA isoform species. This is ...
Nanopore direct RNA sequencing detects differential expression ...
Differential expression analysis enables comparisons between different tissues or conditions to identify genes that play a major role in ...
Latest Direct RNA Sequencing Kit enables higher accuracy and output
Shared in a recent poster, this demonstrated that direct RNA nanopore sequencing can call m6A modifications at single-position resolution with ...
RATTLE: reference-free reconstruction and quantification of ...
Nanopore sequencing enables the efficient and unbiased measurement of transcriptomes. Current methods for transcript identification and ...
NanoTrans: an integrated computational framework for ...
Nanopore direct RNA sequencing (DRS) provides the direct access to native RNA strands with full-length information, shedding light on rich ...
Nanopore direct RNA sequencing detects differential expression ...
Oxford Nanopore Technologies · Optimisation of nanopore sequencing for forensic STR analysis · Identification of m6A RNA modifications at single ...
Nanopore direct RNA sequencing detects differential expression ...
Measurement of expression and fold changes between synthetic control. RNAs confirmed accurate quantification of genes and transcripts. Differential expression ...
RNA and cDNA sequencing | Oxford Nanopore Technologies
Nanopore technology uniquely delivers high outputs of any-length reads that span full-length RNA transcripts, facilitating their accurate, unambiguous analysis ...
How direct RNA nanopore sequencing can enhance your research
... quantify gene and isoform expression, detect RNA modifications and resolve poly-A lengths without PCR bias. In this Knowledge Exchange, you ...
Accurate expression quantification from nanopore direct RNA ...
Median read quality score, 9.8, 10.2 ; Longest alignment length (nt), 13 439, 15 081 ; Reads aligned to genome (%), 97.5, 97.8 ; Reads aligned to transcriptome (%) ...