An in vivo platform to select and evolve aggregation|resistant proteins

An in vivo platform to select and evolve aggregation-resistant proteins

Protein biopharmaceuticals are highly successful, but their utility is compromised by their propensity to aggregate during manufacture and ...

An in vivo platform to select and evolve aggregation-resistant proteins

Using this assay as a directed evolution screen, we demonstrate the generation of aggregation resistant scFv sequences when reformatted as IgGs.

Data associated with 'An in vivo platform to select and evolve ...

Data associated with 'An in vivo platform to select and evolve aggregation-resistant proteins'. Citation. Ebo, Jessica S. and Saunders, Janet ...

Selection and evolution of aggregation resistant proteins

Protein therapeutics are highly effective medicines, but challenges can arise during development and manufacturing which can lead to ...

An in vivo platform for identifying inhibitors of protein aggregation

... An in vivo platform for identifying inhibitors of protein aggregation | Protein ... An in vivo platform to select and evolve aggregation-resistant proteins.

An in vivo platform to select and evolve aggregation-resistant proteins.

An in vivo platform to select and evolve aggregation-resistant proteins. Nat. Commun. Pub Date : 2020-04-14. DOI : 10.1038/s41467-020-15667-1.

An in vivo platform for identifying inhibitors of protein aggregation

Unlike the other test proteins selected, Dp47d rapidly aggregates in vitro into amorphous ... system described here has previously been used to evolve protein ...

'Directing' evolution to identify potential drugs - The Astbury Centre

Read An in vivo platform to select and evolve aggregation-resistant proteins on the Nature Comms website. Social media links. Twitter. Site ...

The effect of mutation on an aggregation-prone protein - PNAS

After evolving the aggregation-prone protein, D76N-β2m, the only mutations able to improve D76N-β2m behavior in vivo involve residues in a ...

High-throughput directed evolution: a golden era for protein science

Innovative screening technologies are emerging for in vivo continuous evolution to enable engineering of proteins with enhanced biophysical ...

High-throughput directed evolution: a golden era for protein science

Innovative screening technologies are emerging for in vivo continuous evolution to enable engineering of proteins with enhanced biophysical properties, as well ...

A Review of Fifteen Years Developing Computational Tools to Study ...

Under this constant and inevitable pressure of aggregation, proteins have evolved ... An in vivo platform to select and evolve aggregation-resistant proteins. Nat ...

The effect of mutation on an aggregation-prone protein - PNAS

After evolving the aggregation-prone protein, D76N-β2m, the only mutations able to improve D76N-β2m behavior in vivo involve residues in a single 7-residue ...

An in vivo platform for identifying inhibitors of protein aggregation

Protein aggregation underlies an array of human diseases, yet only one small-molecule therapeutic targeting this process has been successfully developed to ...

An in vivo selection system with tightly regulated gene expression ...

In vivo selection systems are powerful tools for directed evolution of enzymes. The selection pressure of the systems can be tuned by ...

Using protein engineering to understand and modulate aggregation

Directed evolution allows the identification of aggregation-resistant proteins in the absence of mechanistic understanding. •. Deep mutational ...

Automated Continuous Evolution of Proteins in Vivo

ACE pairs OrthoRep, a genetic system for continuous targeted mutagenesis of user-selected genes in vivo, with eVOLVER, a scalable and automated ...

Prevention of amyloid‐like aggregation as a driving force of protein ...

Prevention of amyloid‐like aggregation as a driving force of protein evolution ... Uncontrolled protein aggregation is a constant challenge in all compartments of ...

Ultrahigh-throughput screening-assisted in vivo directed evolution ...

We demonstrated the success of the in vivo evolution platform with β-lactamase as a model, with an approximately 600-fold increase in the ...

Aggregation is a Context-Dependent Constraint on Protein Evolution

Thus, understanding the relationship between protein evolution and aggregation is an important goal. Here, we analysed two deep mutational scanning experiments ...