Application of Multiplex TaqMan Real|Time PCR Assay in ...

TaqMan Multiplex Real-Time PCR (qPCR) Solution

Successful multiplex qPCR enables the amplification of more than one target in a single reaction using different reporters with distinct fluorescent spectra and ...

Application of Multiplex TaqMan Real-Time PCR Assay in ... - MDPI

Our developed assay showed high specificity, sensitivity, and reproducibility for the simultaneous detection and differentiation of five lily-infecting viruses.

TaqMan™ Assay Multiplex PCR Optimization - APPLICATION GUIDE

Removed TaqMan™ Copy Number Assays. •. Updated compatible Master Mixes, real-time PCR instruments, and calibration plates. •. Updated for ...

Development of a TaqMan-probe-based multiplex real-time PCR for ...

Here, we developed a multiplex TaqMan-probe-based real-time PCR for the simultaneous detection of PEDV, PDCoV, PToV, and SADS-CoV for the first ...

TaqMan probe-based one-step multiplex real-time RT-PCR assay ...

Therefore, in this report, a multiplex TaqMan probe-based one-step RT-qPCR assay simultaneously targeting FMDV 5′UTR and 3Dpol regions, and 18S ...

Multiplex TaqMan real-time PCR assay for high-throughput ...

In this study, a one-tube multiplex TaqMan real-time PCR assay was established for simultaneous detection of the highly toxic mushroom species from three ...

A TaqMan-probe-based multiplex real-time RT-qPCR for ...

TaqMan-probe-based real-time qPCR is a rapid, high specificity, high sensitivity, and great reproducibility detection tool for identification of ...

One-step multiplex TaqMan probe-based method for real-time PCR ...

One-step multiplex TaqMan probe-based method for real-time PCR detection of four canine diarrhea viruses · Abstract. Viral canine diarrhea has ...

A multiplex TaqMan real-time RT-PCR assay for the simultaneous ...

Research article. A multiplex TaqMan real-time RT-PCR assay for the simultaneous detection of H4, H6, and H10 avian influenza viruses.

How to Optimize Multiplex qPCR Experiments -- Ask TaqMan® Ep ...

Submit your real-time PCR questions at http://www.lifetechnologies.com/asktaqman In this video, Sr. Field Applications Specialist Doug Rains ...

Can i use Taqman probe based real time singleplex mastermix for ...

Schematic illustrating the hemi-nested PCR protocol used to measure IFNB1 mRNA levels in single DCs. DCs were directly sorted into 384-well PCR ...

Comprehensive Multiplex One-Step Real-Time TaqMan qRT-PCR ...

Real-time quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) assay is one of the reliable and desirable methods for specific detection and ...

How necessary is it to use a multiplex master mix for ... - ResearchGate

Can I still use the TaqMan Universal PCR Master Mix for the multiplex assays? ... for the Multiplex Real-Time PCR, (2013). http://www.gene ...

Development of a diagnostic assay by three-tube multiplex real-time ...

Results demonstrate the multiplex TaqMan RT-PCR was capable of achieving a sensitive detection for the selected nine pathogens in the range of ...

TaqMan® Multiplex Master Mix | Fisher Scientific

Performing Real-Time PCR Assays. General Guidelines. • Mix the TaqMan® Multiplex Master Mix thoroughly before use by swirling the bottle. • Thaw frozen ...

Development and application of a TaqMan-probe-based multiplex ...

Development and application of a TaqMan-probe-based multiplex real-time PCR assay for simultaneous detection of porcine circovirus 2, 3, and 4 ...

qPCR ProbesMaster UNG lowROX, TaqMan® Detection & Multiplex

Real-time PCR technology based on dual-labeled DNA probes provides a high sensitive and high specific PCR system with multiplexing capability. It requires two ...

Double-Tube Multiplex TaqMan Real-Time PCR for the Detection of ...

The use of universal primers could reduce the competition between primers and DNA templates, and greatly improve the sensitivity. (47) Also, ...

Real-Time PCR Applications Guide - Bio-Rad

Below we describe a multicolor, multiplex real-time PCR assay using TaqMan probes for the simultaneous discrimination of allelic variants of the two most.

A TaqMan-based multiplex real-time PCR assay for the rapid ...

Taken together, the TaqMan-based real-time PCR method established in this study is rapid, sensitive, specific, and is capable of detecting the ...