Aptamer Regulated Transcription for In|vitro Sensing and Transduction

Plug-and-play protein biosensors using aptamer-regulated in vitro ...

Here, we develop a biosensing platform based on aptamer-regulated transcription in which aptamers integrated into transcription templates serve ...

Aptamer Regulated Transcription For In-Vitro Sensing And ...

Particularly, systems and methods utilize a transcription template with an aptamer domain configured to bind a molecule of interest to regulate ...

Aptamer Regulated Transcription for In-vitro Sensing and Transduction

A biomarker detection system, called ARTIST, that utilizes aptamers to detect the binding of proteins, leading to a change in fluorescent signal based on ...

WO2024118806A1 - Aptamer regulated transcription for in-vitro ...

APTAMER REGULATED TRANSCRIPTION FOR IN-VITRO SENSING AND TRANSDUCTION. TECHNICAL FIELD. [0001] The present disclosure relates to nucleic acid transcription ...

APTAMER REGULATED TRANSCRIPTION FOR IN-VITRO ...

... aptamer regulated transcription for in-vitro sensing and transduction (ARTIST). We used ARTIST to design a biosensor for Interferon-gamma (IFN-γ) protein, a ...

Plug-and-play protein biosensors using aptamer-regulated in vitro ...

By coupling aptamer-regulated transcription with diverse transduction circuits, we rapidly construct analog protein biosensors or digital ...

Aptamer-regulated transcription for modular and tunable protein ...

We present Aptamer Regulated Transcription for In vitro Sensing and Transduction (ARTIST), a modular platform based on aptamer-regulated transcription for ...

(PDF) Plug-and-play protein biosensors using aptamer-regulated in ...

By coupling aptamer-regulated transcription with diverse transduction circuits, we rapidly construct analog protein biosensors and digital ...

Aptamers 101: aptamer discovery and in vitro applications in ... - NCBI

Electrochemical aptamer-based (E-AB) sensors are one type of label-free biosensor that employs conformational change of the aptamer upon binding ...

Development of biosensors with aptamers as bio-recognition element

In this work, an RNA aptamer, specific for the protein trans-activator of transcription (Tat) of HIV-1, has been used as bio-recognition element to develop a ...

Aptamer-based regulation of transcription circuits - RSC Publishing

We propose synthetic DNA/RNA transcription circuits based on specific aptamer recognition. By mimicking transcription factor regulation, combined with ...

High-throughput, fluorescent-aptamer-based measurements of ...

However, in vitro mechanistic studies of transcription have lagged with respect to throughput. Here, we describe the use of a real-time, fluorescent-aptamer- ...

In vitro transcription of a fluorescence-activating aptamer rapidly...

| In vitro transcription of a fluorescence-activating aptamer rapidly generates a visible RNA output. a, T7 RNA polymerase transcription of the fluorescence- ...

Thrombin-Mediated Transcriptional Regulation Using DNA ...

The results described here pave the way for the use of DNA aptamers to achieve modular regulation of transcription in response to a wide variety ...

Aptamer-based and aptazyme-based riboswitches in mammalian cells

RNA aptamers for desired ligands can be obtained relatively easily by in vitro selection (SELEX) [5,6]; therefore, riboswitches can potentially ...

Revisiting T7 RNA polymerase transcription in vitro with the Broccoli ...

We have presented a method using the Broccoli RNA aptamer as a direct and rapid fluorescent readout for in vitro transcription that should be straightforward to ...

Anti-Transcription Factor RNA Aptamers as Potential Therapeutics

RNA aptamers make interesting candidates for TF modulation because of their unique characteristics. The products of in vitro selection, aptamers are short ...

An RNA Aptamer that Induces Transcription - Cell Press

We identified an RNA aptamer that induces TetR-controlled gene expression in Escherichia coli when expressed in the cell.

RNA-based transcription activator derived from an inhibitory aptamer

Utilizing a well-characterized site-specific RNA aptamer for TFIIB, we were able to delineate some key features of this process. By rationally converting an ...

Riboswitches: A Common RNA Regulatory Element - Nature

During transcription, the aptamer domain is always the first part of the RNA to be synthesized and fold into a shape capable of binding ligand. After completion ...