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Automating Primary Human T Cell Culture


Automating Primary Human T Cell Culture - Indee Labs

Reliable and effective T cell culture is critical to developing and testing novel CAR-T therapies. It is also a monotonous, error-prone, ...

Automated Expansion of Primary Human T Cells in Scalable and ...

Adoptive immunotherapy is a highly effective strategy for treating many human cancers, such as melanoma, cervical cancer, lymphoma, and leukemia.

Establishing the scalable manufacture of primary human T-cells in ...

Establishing the scalable manufacture of primary human T-cells in an automated stirred-tank bioreactor ... T-flask static culture, with equivalent cell quality.

Automated Expansion of Primary Human T Cells in Scalable and ...

The hydrogel tubes protect cells from hydrodynamic stresses and confine the cell mass less than 400 µm (in radial diameter) to ensure efficient ...

Automated Expansion of Primary Human T Cells in Scalable and ...

A novel method is developed for culturing human T cells. Cells are cultured within microscale alginate hydrogel tubes suspended in cell ...

Piloting a scale-up platform for high-quality human T-cells production

This study explores the growth of primary T-cells sourced from healthy human donors. ... By leveraging automation and precise control over culture ...

Development of an automated manufacturing process for large ...

... Primary Human NK Cells ... 31. Marín Morales, J.M. ∙ Münch, N. ∙ Peter, K. ... Automated Clinical Grade Expansion of Regulatory T Cells in a Fully ...

In vivo human T cell engineering with enveloped delivery vehicles

Main. Therapeutic interventions involving genome editing require the safe and effective delivery of molecules into target cell nuclei. Although ...

Automated, scaled, transposon-based production of CAR T cells

... primary human T cells. Transposition was accomplished with minicircle (MC) ... T cell cultures contained a high percentage of viable T cells. In ...

Protocol Co-culture of primary human T cells with leukemia cells to ...

Alternatives: If available, also the autoMACS® system can be used for automatic T cell isolation. 10. Perform T cell activation using the T Cell ...

Automated production of gene-modified chimeric antigen receptor T ...

... primary T cells in culture systems [14–17]. 14. Kochenderfer, J.N. ... Automated processing: non-transduced T-cell and CAR T-cell cultures.

CellXpress.ai Automated Cell Culture System​ | Molecular Devices

CellXpress.ai - a revolutionary cell culturing system with integrated incubator, liquid handler, and image-based, deep-learning decision-making to automate ...

Automated Cell Culture Systems and Their Applications to Human ...

evaluated the possibility of expanding primary cell lines, that is, human fibroblasts and bone marrow stromal cells (BMSCs), using a benchtop platform with a ...

Development of a robotic cluster for automated and scalable cell ...

A total of 3.0 × 106 primary human T cells in 90 mL of culture medium were allowed to settle for >2 h and then the cells were either tilted manually 10 times at ...

Improved T cell expansion using closed, automated stirred-tank ...

T cell isolation. Primary human T cells from healthy donors were negatively isolated from peripheral blood mononuclear cells (PBMCs) using the Invitrogen ...

Expamers: a new technology to control T cell activation - Nature

To characterize cell phenotype after activation and 7-day culture T cells ... Analysis of TCR activation kinetics in primary human T cells upon ...

Fully automated cell biology workflows - Automata

... Main Menu. LINQ Bench · LINQ Cloud · Service · LINQ · Clinical ... Automated cell culture labs allow instruments to work independently or with minimal human ...

Vectofusin-1 Improves Transduction of Primary Human Cells with ...

The automated T cell ... We compared the soluble peptide, Vectofusin-1, with an established method using recombinant fibronectin to coat cell culture surfaces.

Human Peripheral Blood Pan T Cells, Frozen

Primary human pan-T cells are derived from peripheral blood mononuclear cells using negative separation techniques and ready for downstream usage.

T Cell TransAct™, human | Miltenyi Biotec | USA

T cell activation via CD3 and CD28 with T Cell TransAct™. This stimulation reagent is ready-to-use for in vitro activation and expansion of human T cells.