CRISPR Interference
CRISPR interference (CRISPRi) for sequence-specific control of ...
Sequence-specific control of gene expression on a genome-wide scale is an important approach for understanding gene functions and for ...
CRISPR interference - Wikipedia
CRISPR interference (CRISPRi) is a genetic perturbation technique that allows for sequence-specific repression of gene expression in prokaryotic and eukaryotic ...
CRISPR interference - Horizon Discovery
CRISPR interference (CRISPRi) is a novel method for specific gene knockdown.
CRISPR interference and its applications - PubMed
Sequence-specific control of gene expression is a powerful tool for identifying and studying gene functions and cellular processes. CRISPR interference ...
CRISPR interference (CRISPRi) for sequence-specific control of ...
(a) Depending on the target genomic locus, CRISPRi can block transcription elongation or initiation. When the dCas9-sgRNA complex binds to the nontemplate (NT) ...
Gene Silencing Through CRISPR Interference in Bacteria - Frontiers
Most recently, CRISPR interference (CRISPRi) has been developed as an emerging technology that exploits the catalytically inactive Cas9 (dCas9) ...
CRISPRa and CRISPRi - Synthego
What is CRISPRi? ... CRISPR interference or CRISPRi is also a variant of CRISPR in which a catalytically dead (d) Cas9 is fused with a transcriptional effector to ...
DNA double-strand break-free CRISPR interference delays ... - Nature
Huntington's disease (HD) is caused by a CAG repeat expansion in the huntingtin (HTT) gene. CRISPR-Cas9 nuclease causes double-strand breaks ...
CRISPR Interference - an overview | ScienceDirect Topics
CRISPR can be repurposed to function as an RNA-guided DNA-binding system to interfere with the transcription of an arbitrary gene. This system is called CRISPR ...
Video: What is CRISPR interference or CRISPRi? - Horizon Discovery
Horizon's CRISPRmod CRISPRi system is a novel method for specific gene knockdown at the transcriptional level. Offering a 'gentle' knockdown, ...
CRISPR Plasmids - Repress Gene Expression - Addgene
Catalytically dead dCas9, or dCas9 fused to a transcriptional repressor peptide like KRAB, can knock down gene expression by interfering with transcription.
Quantitative CRISPR interference screens in yeast identify chemical ...
Genome-scale CRISPR interference (CRISPRi) has been used in human cell lines; however, the features of effective guide RNAs (gRNAs) in ...
CRISPR interference-guided multiplex repression of endogenous ...
CRISPRi-mediated multiplex repression of competing pathway genes also resulted in simultaneous reductions of acetate, succinate, lactate, and ...
What is CRISPR interference or CRISPRi? - YouTube
CRISPR interference (CRISPRi) a novel method for specific gene knockdown at the transcriptional level enabling scientists to better ...
CRISPR Interference To Inducibly Repress Gene Expression in ...
A CRISPRi system based on dCas12 is also capable of inducibly repressing incA gene expression in Chlamydia. Given the potentially restrictive NNGRRT PAM ...
Systematic mapping of functional enhancer–promoter connections ...
We use pooled CRISPR screens in combination with CRISPR interference (CRISPRi)—which alters chromatin state at targeted loci through ...
Controlling Gene Expression with CRISPR Interference (CRISPRi)
This article is about repurposing the typical genome editing technology to interfere with gene expression, known as CRISPR interference (CRISPRi). The purpose ...
CRISPR Interference (CRISPRi) for Gene Suppression
CRISPR (cluster of regularly interspaced palindromic repeats) are sequences found in approximately 50% of bacteria and approximately 90% of archaea, ...
Protocol for establishing inducible CRISPR interference system for ...
Protocol for establishing inducible CRISPR interference system for multiple-gene silencing in human pluripotent stem cells · Highlights. Dox- ...
CRISPR interference and its applications - ScienceDirect.com
CRISPR interference (CRISPRi) is an RNA-based method for highly specific silencing of the transcription in prokaryotic or eukaryotic cells. The typical CRISPRi ...