CRISPR Study Reveals Unexpected Roles of Non|Coding RNAs

CRISPR Study Reveals Unexpected Roles of Non-Coding RNAs

CRISPR Study Reveals Unexpected Roles of Non-Coding RNAs · High-Throughput Screening Platform Finds that Distinct Non-Coding RNAs are Essential ...

CRISPR Study Reveals Unexpected Roles of Non-coding RNAs

CRISPR Study Reveals Unexpected Roles of Non-coding RNAs. Laboratory Equipment; South San Francisco (Dec 16, 2016): n/a.

RNA-targeting CRISPR reveals that hundreds of noncoding RNAs ...

The gene editing technology CRISPR has revolutionized biomedical research, with widespread applications from enhancing crop production to ...

CRISPR Study Reveals Unexpected Roles of Non-Coding RNAs

CRISPR Study Reveals Unexpected Roles of Non-Coding RNAs ... Link: https://www.ucsf.edu/news/2016/12/405276/crispr-study-reveals-unexpected-roles- ...

Interrogating lncRNA functions via CRISPR/Cas systems - PMC - NCBI

lncRNAs may be intergenic or intragenic. Intragenic lncRNAs may overlap with protein-coding genes or may be located within the introns of host ...

CRISPR/Cas9 screen uncovers functional translation of cryptic ...

Emerging evidence suggests that cryptic translation within long noncoding RNAs (lncRNAs) may produce novel proteins with important ...

Transcriptome-scale RNA-targeting CRISPR screens ... - Cell Press

This transcriptome-wide survey of functional lncRNAs advances our understanding of noncoding transcripts and demonstrates the potential of ...

CRISPR-based technology to silence the expression of IncRNAs

Other functions of lncRNAs include their ability to form aptamers, which bind and regulate protein complexes (3). The lncRNAs can modulate gene ...

Research reveals the importance of long non-coding RNA ...

The investigators used computational analyses to predict potential polypeptides that could be encoded by known lncRNA molecules, and then they ...

Challenges of CRISPR/Cas9 applications for long non-coding RNA ...

In long non-coding RNA (lncRNA) research, the Cas9 nuclease can delete lncRNA genes or introduce RNA-destabilizing elements into their locus.

Genome-wide Cas9-mediated screening of essential non-coding ...

Here we report a method involving libraries of paired single-guide RNAs targeting both ends of an NCRE as a screening system for the Cas9-mediated deletion of ...

CRISPR links to long noncoding RNA function in mice: A practical ...

This is evident in biomedical research where the ease and low-cost of CRISPR editing has made it the preferred method of manipulating the mouse genome, ...

Stanford explainer: CRISPR, gene editing, and beyond

So, the next time when the virus infects that bacteria cell, the guide RNA rapidly recognizes the virus DNA sequence, binds to it, and destroys ...

CRISPRi-based genome-scale identification of functional long ...

A very small number of lncRNAs have been shown to be functional. Liu et al. carried out a large-scale CRISPR-based screen to assess the function of ∼17,000 ...

Noncoding RNA therapeutics — challenges and potential solutions

The two major classes of ncRNA are the well-studied short microRNAs (miRNAs) and the more recently identified long ncRNAs (lncRNAs) (Box 1).

Targeting long non-coding RNAs in cancer therapy using CRISPR ...

The synergy of lncRNA research and CRISPR-Cas9 technology presents immense potential for individualized cancer treatment, offering renewed hope ...

Non-coding RNAs in human health and disease: potential function ...

Even though ncRNAs were formerly regarded as “evolutionary junk,” new research shows that they substantially impact several molecular pathways.

How CRISPR Is Changing Cancer Research and Treatment - NCI

Scientists design the guide RNA to mirror the DNA of the gene to be edited (called the target). The guide RNA partners with Cas and—true to its ...

Genome-wide CRISPR interference screen identifies long non ...

Although many long non-coding RNAs (lncRNAs) exhibit lineage-specific expression, the vast majority remain functionally uncharacterized in the context of ...

CRISPR Timeline | Broad Institute

They performed small RNA sequencing on Streptococcus pyogenes, which has a Cas9-containing CRISPR-Cas system. They discovered that in addition to the crRNA, a ...