Competitive polymerase chain reaction using an internal standard

A general strategy for the construction of an internal standard for the polymerase chain reaction (PCR) is described together with its application in the ...

Tandem competitive polymerase chain reaction (TC-PCR) - PubMed

Products from each template and internal standard are identified by the band pattern after digestion with the restriction enzyme. As the amount of the ...

Competitive polymerase chain reaction for quantification of ...

The fragment obtained was amplifiable by the FWD and IS primers, thus proving suitable for use as an internal standard. The 372 bp fragment was inserted on a ...

Technical Rapid Construction of Deleted DNA Fragments for Use as ...

quence to produce an internal standard to be used for quantitative competitive. PCR ... Competitive polymerase chain reaction assay for quan- titation of HIV DNA ...

Calibration of the internal standard (IS) by competitive polymerase...

ABSTRACT For competitive polymerase chain reaction (PCR), an internal standard ... The internal standard was calibrated using a quantified culture of ...

Technical Aspects of Quantitative Competitive PCR

This assay is based on competitive co-ampli- fication of a specific target sequence to- gether with known concentrations of an internal standard in one reaction ...

A rapid and versatile method to synthesize internal standards for ...

Competitive PCR is a quantitative adaptation of the PCR method in which a known number of copies of a synthetic mutated internal standard is introduced with ...

A Commentary on the Practical Applications of Competitive PCR

The second type of control is the use of an internal standard. The ... Quantitation of gene copy number and mRNA using the polymerase chain reaction.

(PDF) A rapid and versatile method to synthesize internal standards ...

Using conventional polymerase chain reaction as gold standard method, EasyQ KPC and culture-based molecular tests demonstrated a sensitivity/specificity of ...

Competitive Reverse Transcriptase-Polymerase Chain Reaction ...

They also show the utility and broad application of using synthetic, competitor RNA derived from an expression vector as an internal standard in ...

Quantitative competitive polymerase chain reaction for accurate ...

The quantitative competitive PCR method, developed, is suitable for analysis of clinical specimens and may be particularly valuable for accurate ...

Quantification of mRNA by Polymerase Chain Reaction (PCR) Using ...

Quantification of mRNA by Polymerase Chain Reaction (PCR) Using an Internal Standard and a Nonradioactive Detection Method ... Affiliations: US Army Natick ...

Quantitative Polymerase Chain Reaction Using a Recombinant DNA ...

Using a 308 bp DNA fragment (target DNA) as a template, we have synthesized an internal standard (IS) that is of the same size and uses the ...

Nucleic Acid Quantitation Using the Competitive Polymerase Chain ...

After designing the primers, the rest of the procedure is the same as that for the mutated internal standard construction. In brief, the first ...

Competitive reverse-transcriptase polymerase chain reaction ...

The cell′s own genomic DNA is thereby used as the internal standard. To control for the efficiency of reverse transcription, a standard curve is used in each ...

Quantification of human PPARgamma1 gene expression by ...

The practicality of the competitive PCR method was demonstrated by determining the expression levels of a human transcription factor, ...

Quadruple-Analyte Chemiluminometric Hybridization Assay ...

A 5 μL aliquot from the first PCR mixture was used for amplification of a shorter segment (293 bp) using primers u-innerNK and dNK. The composition of PCR ...

Quantification of white spot syndrome virus DNA through a ...

The competitive PCR was developed by incorporating an internal standard into each. PCR tube. This internal standard is co-amplified with a specific target ...

Comparison of Two Quantitative Polymerase Chain Reaction ...

Five tenfold serially diluted external standards ranging from 1000,000 to 100 copies along with 4 ng of each unknown sample comprised the real-time PCR runs.

The Basics: RT-PCR | Thermo Fisher Scientific - US

At the start of a PCR reaction, reagents are in excess, template and product are at low enough concentrations that product renaturation does not compete with ...