Degron tagging for rapid protein degradation in mice

Importantly, dTAGv1 was shown to promote degradation of certain tagged proteins that were poorly degraded by dTAG-13 (Bondeson et al., 2022; ...

Degron tagging for rapid protein degradation in mice - PubMed

Degron tagging for rapid protein degradation in mice. Dis Model Mech ... Degron tagging allows proteins of interest to be rapidly degraded ...

Degron tagging for rapid protein degradation in mice - PMC

Importantly, dTAGv1 was shown to promote degradation of certain tagged proteins that were poorly degraded by dTAG-13 (Bondeson et al., 2022; ...

Degron tagging for rapid protein degradation in mice

Degron tagging allows proteins of interest to be rapidly degraded, in a reversible and tuneable manner, in response to a chemical stimulus.

Rapid and specific degradation of endogenous proteins in mouse ...

This shows that degradation of endogenous proteins via the AID system is reversible in vivo. Target protein was also efficiently degraded in 2 ...

The auxin-inducible degron 2 technology provides sharp ... - Nature

Proteins can be targeted for rapid degradation by the ubiquitin–proteasome pathway. Protein knockdown can be achieved by recruiting a ...

Scientists generate new targeted protein degradation system that ...

Scientists generate new targeted protein degradation system that tunes a cell's own proteins ... Researchers have engineered a compact degron tag ...

Plasmids 101: Degron Tags - Addgene Blog

The degradation and reversal are rapid compared to other systems because the system modulates protein post-translationally, unlike many other ...

Degron tagging for rapid protein degradation in mice - Ex Libris

Degron tagging allows proteins of interest to be rapidly degraded, in a reversible and tuneable manner, in response to a chemical stimulus. This provides ...

Systematic profiling of conditional degron tag technologies for target ...

For example, GFP and RFLuc fusion proteins were degraded rapidly by most CDTs within 6 h of treatment, while maximal degradation of the ...

Degron tagging for rapid protein degradation in mice - ResearchGate

Request PDF | Degron tagging for rapid protein degradation in mice | Degron tagging allows proteins of interest to be rapidly degraded, in a reversible and ...

Degron Tagging Cluster - MRC National Mouse Genetics Network

... protein-destruction pathways to facilitate rapid, tuneable and reversible degradation (Figure 2). Degron tags are already widely and successfully used in ...

A degron-based approach to manipulate Eomes functions in ... - PNAS

The dTAG system (15, 18) exploits a modified FKBP12 protein, FKBP12F36V, as a “degron tag” to target proteins for degradation. Treatment with a ...

Establishment and characterization of mouse lines useful for ...

... mice ... 3.6 The endogenously tagged protein was effectively degraded via the AID2 system ... Rapid and efficient degradation of endogenous proteins ...

Generation of knock-in degron tags for endogenous proteins in mice ...

Here, we provide a step-by-step protocol for generating genetically engineered mouse (GEM) models harboring a degradation tag (dTAG) fused to ...

Rapid and efficient degradation of endogenous proteins in vivo ...

Here, we tested the functionality of the degradation tag (dTAG) degron system in mammalian development. We generated a homozygous knock-in mouse with a FKBP12 ...

Rapid and specific degradation of endogenous proteins in mouse ...

Auxin-inducible degrons are a chemical genetic tool for targeted protein degradation and are widely used to study protein function in cultured ...

Continuous evolution of compact protein degradation tags ... - Science

Proteins recruited to this degron•small molecule•E3 ligase ternary complex by fusion to a degron tag are degraded by the ubiquitin-proteasome ...

A novel protein knockdown technology: auxin-inducible degron (AID ...

In the degron technology, target protein which is added to the degron sequences (a peptide sequence that induces protein degradation) are removed dependent on ...

Degron tagging for rapid protein degradation in mice - OUCI

Degron tagging for rapid protein degradation in mice. https://doi.org/10.1242 ... ABSTRACT Degron tagging allows proteins of interest to be rapidly degraded ...