Detection of Clostridium perfringens Using Novel Methods Based on ...

Detection of clostridium perfringens using novel methods based on recombinase-aided amplification assay-assisted CRISPR/Cas12a system.

Overall, the constructed C. perfringens detection methods, RAA-CRISPR/Cas12a-FL and RAA-CRISPR/Cas12a-LFS, have great potential as a novel ...

Rapid, simultaneous detection of Clostridium sordellii and ... - PubMed

Rapid, simultaneous detection of Clostridium sordellii and Clostridium perfringens in archived tissues by a novel PCR-based microsphere assay: ...

A novel in situ methodology for visual detection of Clostridium ...

A novel SRCA assay was developed for rapid and visual detection of Clostridium perfringens in meat. •. The analytical sensitivity of the SRCA assay (80 fg) was ...

Extensive genome analysis identifies novel plasmid families in ...

Globally, the anaerobic bacterium Clostridium perfringens causes severe disease in a wide array of hosts; however, C. perfringens strains are also carried ...

A novel in situ methodology for visual detection of Clostridium ...

A novel SRCA assay was developed for rapid and visual detection of Clostridium perfringens in meat. •. The analytical sensitivity of the SRCA assay (80 fg) ...

A novel in situ methodology for visual detection of Clostridium ...

Rapid and on-site detection of C. perfringens is of specific importance in developing countries. In the present study, saltatory rolling circle amplification ( ...

Identification of Novel Clostridium perfringens Type E Strains ... - PLOS

The toxin genes encoded by this plasmid are expressed based upon the results of RT-PCR assays. The ∼65 kb plasmid is closely related to the pCPF4969 cpe plasmid ...

Visual Detection of Clostridium perfringens Alpha Toxin by ... - MDPI

perfringens methods are immunological tests, molecular biological tests, etc. Typical identification is mainly based on the detection methods in the serum ...

Detection and Toxin Typing of Clostridium perfringens in Formalin ...

Since current microbiology methods are not suitable to detect Clostridium perfringens in formalin-fixed, paraffin-embedded tissue samples, we developed a ...

Heliyon - Cell Press

There is a need for a simple, rapid and equipment free detection system for C. perfringens as conventional anaerobic culture method is labour ...

Molecular methods for the analysis of Clostridium perfringens ...

This article describes and evaluates the use of plasmid profiling, ribotyping and PFGE for strain differentiation of C. perfringens from food poisonings and ...

Development of a novel polymerase spiral reaction (PSR) assay for ...

... detection of Clostridium perfringens in meat" by A. Milton et al ... 1995. TLDR. A polymerase chain reaction (PCR) procedure was developed for direct detection of ...

Use of an EZ-Tn5-Based Random Mutagenesis System to Identify a ...

perfringens random mutagenesis approaches. It allowed identification of a novel C. perfringens toxin regulatory locus with homology to the Agr system of S.

Large-Scale Genomic Analyses and Toxinotyping of Clostridium ...

perfringens was the sole pathogen isolated from the incriminated food, the cpe gene was not detected in strains related to 13 outbreaks. This ...

Detection, Identification and Differentiation of Clostridium perfringens

In addition, the recovery of C. perfringens was rejected by ISO in favor of methods based on TSC agar. ... TSC detects all sulphite-reducing clostridia, however, ...

Development of Real-Time PCR for the Detection of Clostridium ...

When the two methods were compared in food samples inoculated with Cl. perfringens, the culture method detected 52 positives, whereas real-time PCR detected 51 ...

Sensitive quantification of Clostridium perfringens in human feces by ...

Rapid and specific detection has been achieved by molecular approaches based on PCR, and it has been used as a reliable tool to detect C.

USDA/FSIS Microbiology Laboratory Guidebook 3rd Edition/1998

In foodborne disease outbreaks, findings of hundreds of thousands or more organisms per gram of food supports a diagnosis of C. perfringens foodborne illness ...

Typing of sheep clinical isolates and identification of enterotoxigenic ...

The aim of this work is to evaluate the PCR technique when used to investigate and type Clostridium perfringens. This method involves the use of toxin gene ...