Detection of Rhythmic Bioluminescence From Luciferase Reporters ...

Detection of rhythmic bioluminescence from luciferase reporters in ...

The unicellular cyanobacterium Synechococcus elongatus PCC 7942 is the model organism for studying prokaryotic circadian rhythms. Although S. elongatus does ...

This chapter describes the luciferase vectors and monitoring devices that we use to identify and characterize elements of the cyanobacterial circadian system.

Detection of rhythmic bioluminescence from luciferase reporters in ...

Detection of rhythmic bioluminescence from luciferase reporters in cyanobacteria. · Plasmids from Article · Antibodies from Article · Sign Up for Our Newsletter.

Detection of Rhythmic Bioluminescence from Luciferase Reporters ...

Detection of Rhythmic Bioluminescence from Luciferase Reporters in Cyanobacteria. Jayna L. Ditty Show author details. Circadian Rhythms: Methods and Protocols.

Detection of rhythmic bioluminescence from luciferase reporters in ...

A choice of methods and equipment to detect light production from the luciferase fusions provides a means for high-throughput, automated mutant screens as ...

Detection of rhythmic bioluminescence from luciferase reporters in ...

elongatus chromosome. A choice of methods and equipment to detect light production from the luciferase fusions provides a means for high-throughput, automated ...

Real-Time Luminescence Reporting of Circadian Gene Expression ...

Luminescence reporters have been used successfully in studies of circadian rhythms ... luciferase reporter is extremely weak, several CCD cameras can detect the ...

Methods for Detecting PER2:LUCIFERASE Bioluminescence ...

... reporter, such as bioluminescence from the reporter gene luciferase controlled by a rhythmically expressed gene of interest. Here we describe methods that ...

Methods for Detecting PER2:LUCIFERASE Bioluminescence ...

In their work, rhythmic signal was detected from bioluminescent reporters directed to the liver using viral transduction in hairless mice, as well as in mPer2 ...

LUCIFERASE bioluminescence rhythms in freely moving mice

Further, we demonstrate that a synthetic luciferase substrate, CycLuc1, can support circadian rhythms of bioluminescence, even when delivered at ...

PERIOD2::LUCIFERASE real-time reporting of circadian dynamics ...

To monitor circadian dynamics in different tissues, luminescence was continuously measured in real time with PMT detectors. ... rhythms of bioluminescence.

LUCIFERASE bioluminescence rhythms in freely moving mice ...

Further, we demonstrate that a synthetic luciferase substrate, CycLuc1, can support circadian rhythms of bioluminescence, even when delivered at ...

Bioluminescence imaging in living organisms - ScienceDirect.com

Luciferase activity is now used to detect protein–protein interactions, to track cells in vivo, and to monitor transcriptional and post-transcriptional ...

Methods for Detecting PER2:LUCIFERASE Bioluminescence ...

An important tool for studying cellular and tissue circadian rhythms is the use of a gene reporter, such as bioluminescence from the reporter ...

Single-Cell Bioluminescence Imaging System for Monitoring ...

Owing to its non-invasive nature and quantitative performance, bioluminescence monitoring using luciferase reporter genes is a major tool for analyzing the ...

Harnessing bioluminescence for drug discovery and epigenetic ...

By inserting luciferase genes into specific sites in the genome, it is possible to engineer reporters to monitor gene expression in its native context, and to ...

luciferase as a reporter of circadian gene expression in cyanobacteria.

Therefore, the rhythms of Synecho- coccus satisfy the three fundamental criteria for circadian rhythms. Does the rhythm of bioluminescence in AMC149 prove that.

Methods for detecting PER2::LUCIFERASE bioluminescence ...

Using a LumiCycle In Vivo (Actimetrics), we determined conditions that allow detection of circadian rhythms of bioluminescence from the PER2 reporter, PER2::LUC ...

Bioluminescent and Fluorescent Reporters in Circadian Rhythm ...

A light detector can measure more than 108-fold changes in the amount of luciferase (Promega Corporation, 2000). (3) Adequate instability. Real- ...

Luciferase Reporters in Circadian Research - YouTube

This video was made by the BioClock Studio for use in an upper division undergraduate class on circadian rhythms and biological clocks at UC ...