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Development of a high sensitivity RT|PCR assay for detection of ...


Development of a high sensitivity RT-PCR assay for detection of ...

The COVID-19 pandemic requires sensitive detection of the SARS-CoV-2 virus from samples to ensure accurate detection of infected patients, ...

Development of a Real-Time PCR Assay for Detection of ...

PCR-based assays have various advantages over microscopy and RDT: they are highly specific and are capable of high sensitivity (6, 20, 23, 27), and as few as ...

Development of a universal real-time RT-PCR assay for detection of ...

... testing capacity to improve prognosis. The universal RT-qPCR assay for pan-SARS-CoV diagnosis demonstrated high sensitivity and specificity ...

Development and evaluation of a high-sensitivity RT-PCR lateral ...

This study aimed to develop and evaluate a simplified, high-sensitivity assay for early HIV-1 detection before seroconversion.

Development of highly sensitive one-step reverse transcription ...

... RT-qPCR assays for early detection and higher sensitivity compared to two-step RT-qPCR. 2. Materials and methods. 2.1. Wastewater sampling.

Development of a qualitative real-time RT-PCR assay for the ...

Development of a qualitative real-time RT-PCR assay for the detection ... development of a highly specific and sensitive assay. Primer ...

Development and Evaluation of a High-Sensitivity RT-PCR Lateral ...

This study aimed to develop and evaluate a simplified, high-sensitivity assay for early HIV-1 detection before seroconversion. The assay ...

Development of a high sensitivity TaqMan-based PCR assay for the ...

A new primer/probe pair for TaqMan based qPCR assay, was developed, optimized, and validated for the sensitive and specific detection of TB among various ...

Development and Validation of a Real-Time PCR Assay for Rapid ...

The assay was highly sensitive, with a detection limit of 1 C. auris CFU/PCR. The high sensitivity of the real-time PCR assay is not surprising since the ...

Development of multiplex real-time RT-PCR assay for the detection ...

... polymerase chain reaction (RT-PCR) is a sensitive assay ... detect SARS-CoV-2 infection: high sensitivity and increased testing capacity.

Development and performance evaluation of the first in-house ...

Development and performance evaluation of the first in-house multiplex rRT-PCR assay in Bangladesh for highly sensitive detection of SARS-CoV-2. Author links ...

(PDF) Development of a high sensitivity RT-PCR assay for detection ...

We demonstrate that the Erba Molecular ErbaMDx SARS-CoV-2 RT-PCR Kit (EM kit) delivers high sensitivity, achieving analytical detection of 5 ...

Three Novel Real-Time RT-PCR Assays for Detection of COVID-19 ...

These rRT-PCR assays reported here could be used for laboratory diagnosis of COVID-19 infection with high sensitivity, specificity, and applicability.

Development and Evaluation of Novel and Highly Sensitive Single ...

This design combines the convenience of real-time RT-PCR with the high specificity and sensitivity of nested PCR. ... PCR assay for highly sensitive detection of ...

Heliyon

Development and evaluation of a high-sensitivity RT-PCR lateral flow assay for early detection of HIV-1 infection. Supachai Sakkhachornphopa ...

Development of a reverse transcription droplet digital PCR (RT ...

... (RT-ddPCR) assay for sensitive detection of simian immunodeficiency virus (SIV) ... analysis of multiple targets, and increased sensitivity ...

Development of RT-PCR Based Diagnosis of SARS-CoV-2

The qRT-PCR has the benefits of high sensitivity, high precision, and a wide ... sensitivity of PCR assay [67]. One study attempted to deduce the ...

Reconstructed diagnostic sensitivity and specificity of the RT-PCR ...

This study was aimed at the estimation of diagnostic sensitivity and specificity of the first RT-PCR test developed by China CDC in January 2020.

Development, Evaluation, and Standardization of a Real-Time ...

The best performance in terms of sensitivity and reproducibility was achieved by a one-step TaqMan RT-PCR, with a sensitivity enabling the detection of 0.05 ...

Development of a qualitative real-time RT-PCR assay for the ...

Development of a qualitative real-time RT-PCR assay for the detection of ... high specificity for COVID-19. Later empiric testing of clinical samples ...