Development of a robust TaqMan probe|based one|step multiplex ...

Development of a robust TaqMan probe-based one-step multiplex ...

In this study, we developed a novel in-house made multiplex RT-qPCR assay, with high sensitivity, specificity, and reliability for the ...

Development of a robust TaqMan probe-based one-step multiplex ...

In this study, we developed a novel in-house made multiplex RT-qPCR assay, with high sensitivity, specificity, and reliability for the diagnosis of SARS-CoV-2 ...

Development of a robust TaqMan probe-based one-step multiplex ...

The sensitivity of the multiplex RT-qPCR for detection of the SARS-CoV-2 and Influenza A and B genomes was consistent with previously reported ...

Development of a robust TaqMan probe-based one-step multiplex ...

The method offers high analytical sensitivity, reliability, specificity, and accuracy. Its use will streamline testing for co-infections, increase testing ...

Development of a robust TaqMan probe-based one-step multiplex ...

Development of a robust TaqMan probe-based one-step multiplex RT-qPCR for Simultaneous detection of SARS-. CoV-2 and in uenza A/B viruses.

Development of a robust TaqMan probe-based one-step multiplex ...

The diagnostic sensitivity of the multiplex RT-qPCR was 94.4%, 93.7%, and 100% for the detection of SARS-CoV-2, Influenza A, and Influenza B genomes, ...

Development of a TaqMan probe-based multiplex real-time PCR for ...

... one reaction. Remarkably, the assay has good specificity and no ... Highly sensitive and multiplexed one-step RT-qPCR for profiling genes involved ...

Peer Review reports - BMC Microbiology

Peer Review reports. From: Development of a robust TaqMan probe-based one-step multiplex RT-qPCR for simultaneous detection of SARS-CoV-2 and Influenza A/B ...

TaqMan probe-based one-step multiplex real-time RT-PCR assay ...

Further, the multiplex RT-qPCR assay proved to be robust, showing an inter-assay co-efficient of variations ranging from 1.3% to 3.03% for FMDV- ...

Development of a robust TaqMan probe-based one-step multiplex ...

Development of a robust TaqMan probe-based one-step multiplex RT-qPCR for simultaneous detection of SARS-CoV-2 and Influenza A/B viruses. Citation metadata.

Development of a robust TaqMan probe-based one-step multiplex ...

Development of a robust TaqMan probe-based one-step multiplex RT-qPCR for Simultaneous detection of SARS-CoV-2 and influenza A/B viruses.

Development of a robust TaqMan probe-based one-step multiplex ...

Title. Development of a robust TaqMan probe-based one-step multiplex RT-qPCR for simultaneous detection of SARS-CoV-2 and Influenza A/B viruses. Authors ...

Development of a TaqMan-probe-based multiplex real-time PCR for ...

... reliable LOD. Specificity of the multiplex real-time PCR assay. To ... Hu H, Jung K, Wang Q, et al. Development of a one-step RT-PCR assay ...

Development and evaluation of one-step RT-qPCR TaqMan ...

Target genes CP, HC-Pro, RdRp, TGB1 and Assay ID used in multiplex panels MP1, MP2, MP3 and MP4. Virus or control, Target gene, Primers Probes, Sequences(5'–3') ...

Development and evaluation of one-step RT-qPCR TaqMan ...

In this study we aimed to rethink current assay designs to facilitate a more robust and more efficient multiplex strategy. We used synthetic.

[PDF] TaqMan probe-based one-step multiplex real-time RT-PCR ...

single reaction tube was developed and evaluated and proved to be robust. Effective control and monitoring the spread of foot-and-mouth disease (FMD) relies ...

Development and validation of cost-effective one-step multiplex RT ...

TaqMan probe-based commercial real-time (RT) PCR kits are expensive but most frequently used in COVID-19 diagnosis.

TaqMan multiplex real-time PCR | Thermo Fisher Scientific

These probes can be ordered with Applied Biosystems™. FAM™ and VIC™ dyes and also with the ABY and JUN dyes, allowing amplification of up to 4 targets in a.

Development of the first PVM TaqMan® primer set and a one-step ...

Reasons for this reduction might prevalently be the strong interaction between primers and probes in the multiplex (Pan et al. 2018) ...

Taqman® probe based multiplex RT-PCR for simultaneous ...

In order to improve the effectiveness of the developed method, a culturing step ... One of these is that the fast and reliable testing methods for specific ...