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Direct|RT|qPCR Detection of SARS|CoV|2 without RNA Extraction ...


Direct-RT-qPCR Detection of SARS-CoV-2 without RNA Extraction ...

The Direct-One-Step-RT-qPCR (DIOS-RT-qPCR) assay detects severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in less than one hour.

Direct RT-qPCR detection of SARS-CoV-2 RNA from patient ... - PLOS

Direct RT-qPCR detection of SARS-CoV-2 RNA from patient nasopharyngeal swabs without an RNA extraction step. Emily A. Bruce, Meei-Li Huang ...

Direct-RT-qPCR Detection of SARS-CoV-2 without RNA ... - PubMed

Direct-RT-qPCR Detection of SARS-CoV-2 without RNA Extraction as Part of a COVID-19 Testing Strategy: From Sample to Result in One Hour · Authors.

SARS-CoV-2 detection by direct rRT-PCR without RNA extraction

In this study, we compared direct rRT-PCR method (without RNA extraction) using SeeGene AllplexTM 2019-nCoV rRT-PCR with the RealStar® SARS-CoV-2 rRT-PCR kit ( ...

Direct diagnostic testing of SARS-CoV-2 without the need for prior ...

However, the majority of these assays require multiple steps including extended sample pretreatments, and/or RNA extraction and subsequent ...

SARS-CoV-2 Direct Detection Without RNA Isolation With Loop ...

In this work, we propose a direct method without RNA extraction, based on the Loop-mediated isothermal amplification (LAMP) and Clustered Regularly Interspaced ...

DIRECT RT-qPCR DETECTION OF SARS-CoV-2 RNA ... - bioRxiv

DIRECT RT-qPCR DETECTION OF SARS-CoV-2 RNA FROM PATIENT NASOPHARYNGEAL SWABS WITHOUT AN RNA EXTRACTION STEP. View ORCID ProfileEmily A. Bruce ...

Detection of SARS-CoV-2 RNA by direct RT-qPCR on ... - PLOS

We aimed to develop a protocol for direct RT-qPCR to detect SARS-CoV-2 in nasopharyngeal swabs without RNA extraction.

Sensitive extraction-free SARS-CoV-2 RNA virus detection using a ...

Detection of SARS-CoV-2 RNA by direct RT-qPCR on nasopharyngeal specimens without extraction of viral RNA. PLoS One, 15 (2020), p. e0236564 ...

Massive and rapid COVID-19 testing is feasible by extraction-free ...

Currently, the widely used method of SARS-CoV-2 detection in clinical diagnostics is an RT-PCR assay, detecting the presence of viral RNA in ...

Analytical and Clinical Validation for RT-qPCR Detection of SARS ...

We present the validation results for an RT-qPCR protocol without prior RNA extraction. Due to its simplicity, this protocol is suitable for widespread ...

RT-qPCR DETECTION OF SARS-CoV-2 RNA FROM ... - bioRxiv

... without the need for an RNA extraction step of any kind. Collectively, our findings provide viable options to circumvent supply chain issues ...

Powerful, versatile SARS-CoV-2 detection - Takara Bio

... SARS-CoV-2 detection. Direct viral detection from crude biological samples—without RNA extraction. Our direct RT-qPCR mixes may be used directly with many ...

Emergency SARS-CoV-2 variants of concern: rapidly direct RT ...

This novel direct RT-qPCR diagnosis method without RNA extraction is a reliable and high-throughput alternative method that can significantly save cost, labor, ...

Comparison of SARS-CoV-2 indirect and direct RT-qPCR detection ...

An attractive option is direct detection from patient samples without RNA extraction, as it increases throughput, decreases costs and ...

(PDF) Direct-RT-qPCR Detection of SARS-CoV-2 without RNA ...

This optimised protocol allows for the direct use of swab transfer media (14 μL) without the need for RNA extraction, achieving comparable ...

Direct-RT-qPCR Detection of SARS-CoV-2 without RNA Extraction ...

The Direct-One-Step-RT-qPCR (DIOS- RT-q PCR) assay detects severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in less than one hour while ...

Direct Detection of SARS-CoV-2 from front nose or saliva without the ...

Direct detection without the need of RNA isolation in a lab – easy workflow, low cost, higher effectivity in less time; Significant time reduction – new FAST ...

SARS-CoV-2 Direct Real Time PCR without RNA Extraction

This includes sample collection in viral transport medium (VTM), viral RNA extraction followed by detection of virus using fluorescence dye- ...

Direct Viral RNA Detection of SARS-CoV-2 and DENV in Inactivated ...

... 2, this study evaluated alternative workflows for SARS-CoV-2 and DENV-2 RNA detection with and without either virus inactivation or nucleic acid extraction.