Elimination of autofluorescence background from fluorescence ...

Here we present a method for reducing the autofluorescence problem by utilizing an azadioxatriangulenium (ADOTA) dye with a fluorescence lifetime of ...

Learn how to Remove Autofluorescence from your Confocal Images

In this case, fluorescence can easily be removed by thorough rinsing with buffered saline or the careful use of solvents. If you are fixing cells, the fixation ...

Tips to Minimize Autofluorescence - FluoroFinder

How to Reduce Autofluorescence · Change the Fixation Method · Remove Red Blood Cells · Eliminate Dead Cells and Debris · Reduce the Concentration of ...

Elimination of autofluorescence background from fluorescence ...

Elimination of autofluorescence background from fluorescence tissue images by use of time-gated detection and the AzaDiOxaTriAngulenium (ADOTA) ...

How to Reduce Autofluorescence - Southern Biotech

Autofluorescence is a term used to describe the background fluorescence present in biological samples which cannot be directly attributed to the fluorophore- ...

Can anyone recommend the best way to remove/reduce ...

The autofluorescence can be caused by quite a big number of natural fluorophores (most common are collagen or elastin for example), so it is I ...

How to reduce autofluorescence | Proteintech Group

Autofluorescence describes background fluorescence in a tissue that is not ... To negate these effects, Sudan black B, a lipophilic dye, can effectively eliminate ...

How can we reduce the autofluorescence or background ...

Try blocking your tissues for a longer period of time (1 hour tends to work pretty well for our lab), using a lower concentration of secondary antibody, or ...

What steps can I take to minimize the impact of autofluorescence ...

Using narrow band emission filters or spectral unmixing can help to remove the autofluorescence of your image. Strive to optimize a protocol where you can ...

How to Reduce Autofluorescence | Labcompare.com

What is autofluorescence? ... Background fluorescence in a tissue that is not attributed to the specific staining of an antigen-antibody- ...

5 Tips To Reduce Autofluorescence - Boster Bio

5 Tips To Reduce Autofluorescence · Use a lower concentration of FCS in the staining buffer · Remove dead cells & other debris · Lyse RBCs properly ...

Autofluorescence: Causes and Cures

There's nearly always some. "background" fluorescence (for unknown reasons) in anything, especially with a broad-band blue excitation. Narrow-band filters and ...

How to reduce autofluorescence in cell-based assays - BMG Labtech

One possibility to circumvent cell-derived autoﬂuorescence is to use red-shifted ﬂuorophores that emit at higher wavelengths and avoid the blue-green region for ...

Reduction of background autofluorescence in brain sections ...

Background fluorescence was notably reduced by immersion of free-floating brain tissue sections in a solution of sodium borohydride (NaBH 4 , 0.1%).

Reduced background autofluorescence for cell imaging using ...

Background autofluorescence is a major issue for the bio-imaging of cells and tissues. The natural emission of light by molecules that increase ...

Tech Tip: Battling Tissue Autofluorescence - Biotium

However, fluorescence detection in tissue samples can be hindered by strong background fluorescence. Autofluorescence can arise from exogenous and ...

Reduce Tissue Autofluorescence and Dramatically Enhance Signal ...

Autofluorescence is background fluorescence due to naturally occurring substances which can affect your signal-to-noise ratio. It can arise ...

An Alternative to Dye-Based Approaches to Remove Background ...

This technique is then compared against the traditional, dye-based autofluorescence sequestration method using Sudan Black B (SBB). Spectral ...

What is Autofluorescence? A Quick Explainer for Biologists

It's background fluorescence in cells or tissues that have not been stained with a specific fluorescent marker. To reduce it, select ...

Simple Method for Reduction of Autofluorescence in Fluorescence ...

Background fluorescence can be notably reduced or eliminated by irradiation with light before treatment with fluorescence probes.