Enrichment protocols for human conjunctival extracellular vesicles ...

Atomic Force Microscopy showed EVs with a slightly ellipsoidal morphology. EVs enriched with UF-precipitation method were further analyzed, ...

Protein enrichment - Latest research and news - Nature

Enrichment protocols for human conjunctival extracellular vesicles and their characterization · Biofunctionalized dissolvable hydrogel microbeads enable ...

Enrichment, Characterization, and Proteomic Profiling of Small ...

While extracellular vesicles (EV), specifically small EVs (sEV), have been proposed to support LEPC homeostasis, data on sEV derived from limbal ...

Isolation and Characterization of Human Conjunctival Mesenchymal ...

The purpose of this study was to isolate and culture human conjunctival mesenchymal stromal cells (Conj-MSCs) from cadaveric donor tissue, ...

Stem Cells Translational Medicine | Oxford Academic

Conjunctival reconstruction via enrichment of human conjunctival epithelial ... Mesenchymal stromal cells-derived small extracellular vesicles protect against UV- ...

Human conjunctiva organoids to study ocular surface homeostasis ...

Finally, conjunctival organoids can be transplanted. Together, human conjunctiva organoid cultures enable the study of conjunctival (patho)- ...

Epithelial Cell-Derived Extracellular Vesicles Trigger the ... - MDPI

The EVs were extracted from the conditioned media (after starving) of corneal epithelial (hTCEpi) and conjunctival (HCjE-Gi) cell lines using ...

Enrichment, Characterization, and Proteomic Profiling of Small ...

Enrichment, Characterization, and Proteomic Profiling of Small Extracellular Vesicles Derived from Human Limbal Mesenchymal Stromal Cells and ...

Extracellular Vesicle Protocol - Sigma-Aldrich

This protocol that follows was designed for enrichment of extracellular vesicles (EVs) from serum-free cell culture media.

Interaction network of extracellular vesicles building universal ...

Using universal analysis in combination with the Human Protein Atlas consensus dataset, we found the genesis of tear EV proteins with 37 tissues ...

Protocol for the separation of extracellular vesicles by ... - Europe PMC

Extracellular vesicles (EVs) play key roles in transporting key molecular constituents as cargo for extracellular trafficking. While several approaches have ...

Biological properties of extracellular vesicles and their physiological ...

Galectin-4 has been detected in EVs secreted by human colorectal cell line HT 29 (61) and colon tumour cell line LIM1215 (28), while galectin-5 ...

Cellular microenvironment of pterygia (A) cell type enrichment ...

We explored the therapeutic efficacy of Mesenchymal stromal cells-derived extracellular vesicles (MSC-EVs) and its inhibition of the functions of dendritic ...

Human Conjunctival MSCs produce extracellular vesicles ... - IOVS

Purpose : To isolate extracellular vesicles (EVs) from human conjunctival mesenchymal stromal cells (Conj-MSCs) and analyze their effect on conjunctival ...

Enhanced enrichment of extracellular vesicles for laboratory and ...

Results · Isolation of EVs from human and porcine serum using NTI-EXO · High-yield EV precipitations along with albumin and immunoglobulins did ...

Enrichment of extracellular vesicles from tissues of the central ...

In the current study, we used an optimized PROSPR protocol to enrich EVs from human and mouse CNS tissues (PROSPR-CNS-EVs), thereby obtaining a ...

Volume 9 Issue 11 | Stem Cells Translational Medicine

... extracellular vesicles. Kian F. Eichholz and others. Stem Cells ... Conjunctival reconstruction via enrichment of human conjunctival epithelial ...

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1. Enrichment, Characterization, and Proteomic Profiling of Small Extracellular Vesicles Derived from Human Limbal Mesenchymal Stromal Cells and ...

OUCI

... enrichment protocols to enhance the yield of LEPC. ... It also consists of the emerging role of exosomes and extracellular vesicles in corneal infections and ...

An optimized protocol for the enrichment of small vesicles from ...

We have demonstrated the use of the present protocol for EV enrichment from mouse and non-human primate heart. The latter lack biochemical ...