Evaluation of general 16S ribosomal RNA gene PCR primers for ...

16S ribosomal RNA gene (rDNA) amplicon analysis remains the standard approach for the cultivation-independent investigation of microbial ...

Based on this evaluation a selection of 'best available' primer pairs for Bacteria and Archaea for three amplicon size classes (100–400, 400–1000, ≥1000 bp) ...

[PDF] Evaluation of general 16S ribosomal RNA gene PCR primers ...

The results of this study may be used as a guideline for selecting primer pairs with the best overall coverage and phylum spectrum for specific applications ...

Evaluation of general 16S ribosomal RNA gene PCR primers for ...

Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencing-based diversity studies. · Archaea · Bacteria · Biodiversity ...

Evaluation of general 16S ribosomal RNA gene PCR primers for ...

Full display result ·, Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencing-based diversity studies · Locations ...

Evaluation of general 16S ribosomal RNA gene PCR primers for ...

16S ribosomal RNA gene (rDNA) amplicon analysis remains the standard approach for the cultivation-independent investigation of microbial diversity.

Optimizing PCR primers targeting the bacterial 16S ribosomal RNA ...

Targeted amplicon sequencing of the 16S ribosomal RNA gene is one of the key tools for studying microbial diversity. The accuracy of this ...

Evaluation of general 16S ribosomal RNA gene PCR primers ... - Scite

Abstract: 16S ribosomal RNA gene (rDNA) amplicon analysis remains the standard approach for the cultivation-independent investigation of microbial diversity ...

Questions about V3–V4 primers for 16S rRNA amplicon sequencing ...

Klindworth, A., Pruesse, E., Schweer, T., Peplies, J., Quast, C., Horn, M., & Glöckner, F. O. (2013). Evaluation of general 16S ribosomal RNA ...

Evaluation of PCR conditions for characterizing bacterial ... - Nature

Full-length bacterial 16S rRNA genes have historically been sequenced using conventional molecular cloning and Sanger sequencing, but this ...

Evaluation of 16S rRNA Gene Primer Pairs for Monitoring Microbial ...

Primers for the amplification of the archaeal and bacterial 16S rRNA genes were selected in a way to avoid amplification of sequences outside the designated ...

In silico evaluation and selection of the best 16S rRNA gene primers ...

This paper analyses these primers using two databases containing 16S rRNA sequences from bacteria and archaea found in the human mouth and describes some of ...

Assessment of 16S rRNA gene primers for studying bacterial ...

Selection of optimal primer pairs in 16S rRNA gene sequencing is a pivotal issue in microorganism diversity analysis.

Critical Evaluation of Two Primers Commonly Used for Amplification ...

We report here an analysis and reevaluation of commonly used primers for amplifying the DNA between positions 27 and 1492 of bacterial 16S rRNA genes.

Direct 16S rRNA-seq from bacterial communities: a PCR ... - Nature

To overcome these drawbacks we propose an approach of direct sequencing of 16S ribosomal RNA without any primer- or PCR-dependent step. The ...

Primer, Pipelines, Parameters: Issues in 16S rRNA Gene Sequencing

Moreover, we evaluated the influence of specific primers and their comparability in a large population-based cohort (n = 1,976 subjects).

Evaluation of primers and PCR conditions for the analysis of 16S ...

Polymerase chain reaction (PCR)-based analysis of 16S rRNA genes is a powerful and essential tool for studies of bacterial diversity, community structure, ...

Evaluation of 16S rRNA Gene Primer Pairs for Monitoring Microbial ...

Evaluation of 16S rRNA Gene Primer Pairs for Monitoring Microbial Community Structures Showed High Reproducibility within and Low Comparability between Datasets ...

Review and re-analysis of domain-specific 16S primers

Here we re-assess the specificity of commonly used 16S rRNA gene primers and present these data in tabular form designed as a tool to aid simple analysis, ...

Evaluation of three PCR primers based on the 16S rRNA gene for ...

Partial sequencing of 16S ribosomal RNA (rRNA) gene is commonly used for the identification of lactic acid bacteria (LAB). The present study evaluated the ...