Exploring effect of culture condition on human retinal progenitor cells

Conclusions : KOSR is known to improve proliferation and maintain stemness of embryonic cells and our experiments suggest that hRPCs diverge into two distinct ...

Comparison of human retinal progenitor cells cultured in media with ...

Moreover, retinal mesenchymal-like stem cells were isolated and identified from the S-hRPCs cultures. Our study demonstrated the potential of retinal MSCs for ...

Medium Depth Influences O2 Availability and Metabolism in Human ...

Retinal pigment epithelium (RPE) oxidative metabolism is critical for normal retinal function and is often studied in cell culture systems. Here ...

Bankable human iPSC-derived retinal progenitors represent a ...

Thawed and passed hiRPCs maintained biochemical and transcriptional RPC phenotypes and their ability to differentiate into all retinal cell ...

Enriched Cultures of Retinal Cells From BJNhem20 Human ... - IOVS

After 1 month, the neuro-retinal progenitor clusters located at the center of pigmented retinal patches were picked and cultured as suspended neurospheres in ...

Organ Cultures for Retinal Diseases | Neuroscience - Frontiers

However, compared to the cultivation of cornea models, retinal organ cultures are more difficult to maintain, mainly due to the different cell ...

Single-cell analyses reveal transient retinal progenitor cells in the ...

Together, these comprehensive single cell analyses shed light on the molecular mechanisms governing human retinal development and provide ...

Human retinal model systems: Strengths, weaknesses, and future ...

Explant cultures allow for isolation of specific retinal regions. •. Retinospheres contain microglia and can be cultured for long periods of time. •. Organoids ...

Engineering Retina from Human Retinal Progenitors (Cell Lines)

In this study, we have explored a spontaneously immortalized human retinal progenitor cell line for its potential in retinal engineering using rotary cultures.

Exploration of physical and chemical cues on retinal cell fate

... effect of the topography and culture medium on dissociated retinal cells. ... human retinal progenitor cell differentiation and transplantation. J. Biomater ...

Exploring Stem-Cell-Based Therapies for Retinal Regeneration - MDPI

Furthermore, BMSCs can produce neurotrophic factors that support cell survival and exert anti-inflammatory effects. An additional benefit of CD34+ cells is ...

PHLDA1-PRDM1 mediates the effect of lentiviral vectors on fate ...

Retinal organoids were derived from the human embryonic stem cells (hESCs). Initially, hESCs were maintained on Vitronectin (VTN-N) matrix with ...

Comprehensive characterization of fetal and mature retinal cell ...

Characterizing cell identity in complex tissues such as the human retina is essential for studying its development and disease.

Stem Cell Reports

Cell culture and retinal organoid generation. Human induced pluripotent stem cells. HPSI0314i-hoik_1 (RRID:CVCL_AE82) was obtained from ECCAC ...

The Influence of Electrical Stimulation on the Proliferation and ...

Results: RPCs in ES group exhibited higher cell densities and viability than the cells in control group without ES (p<0.05). The relative ...

Comparison of human retinal progenitor cells cultured in media with ...

Moreover, retinal mesenchymal-like stem cells were isolated and identified from the S-hRPCs cultures. Our study demonstrated the potential of ...

Stem cells: past, present, and future

... culture conditions. Nat ... Human embryonic stem cells extracellular vesicles and their effects on immortalized human retinal Müller cells.

An Update on Stem Cell Therapy for Retinal Diseases

They can be found in adult bone marrow, adipose tissue, and many other organs. Human bone marrow contains both CD34+ stem cells and MSCs, which ...

Methods for culturing retinal pigment epithelial cells

human pluripotent stem cell-derived retinal pigment epi- thelial cells. ... Retinal pigment epithelial cell differentiation in vitro: influence of culture medium.

Improving the human relevance of cell culture using animal-free ...

Cells can require a 'weaning' period or protocol optimisation to adapt to serum-free or animal-free conditions, and this has been successfully ...