Finding Function for Noncoding RNAs Using a New Kind of CRISPR

Using CRISPR technology that targets RNA instead of DNA, researchers at New York University and the New York Genome Center searched across the ...

CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

Scientists have determined the function of many protein-coding genes thanks to systems like CRISPR/Cas9 that allow them to manipulate full genes ...

CRISPR Gene Editing News - ScienceDaily

Finding Function for Noncoding RNAs Using a New Kind of CRISPR. Nov. 7, 2024 — Genes contain instructions for making proteins, and a central dogma of biology ...

Finding Function for Noncoding RNAs Using a New Kind of CRISPR

RNA-targeting CRISPR reveals that hundreds of noncoding RNAs are essential—not “junk”— including in human development and cancer.

Functional knockout of long non-coding RNAs with genome editing

CRISPR-based genome editing is the most widely used for inactivating lncRNA function at the genomic level. Knocking out the lncRNA function can ...

AI and CRISPR Precisely Control Gene Expression - NYU

Researchers at NYU and the New York Genome Center created a platform for RNA-targeting CRISPR screens using Cas13 to better understand RNA ...

Putting Non-coding RNA on Display with CRISPR - PMC

... different types of non-coding RNAs (ncRNAs) to . ... This finding shows that a ... method to identify proteins involved with functional lncRNA domains.

Researchers uncover new CRISPR-like system in animals that can ...

The first RNA-guided DNA-cutting enzyme found in eukaryotes, Fanzor could one day be harnessed to edit DNA more precisely than CRISPR/Cas ...

New kind of CRISPR technology to target RNA, including RNA ...

The researchers capitalized on a recently characterized CRISPR enzyme called Cas13 that targets RNA instead of DNA. Using Cas13 ...

Full article: Interrogating lncRNA functions via CRISPR/Cas systems

Long noncoding RNAs (lncRNAs) are an increasing focus of investigation due to their implications in diverse biological processes and disease.

CRISPR links to long noncoding RNA function in mice: A practical ...

Here, CRISPR genome editing components are updated for elucidating lncRNA function in mice. Various strategies are highlighted for understanding the function of ...

CRISPRi-based genome-scale identification of functional long ...

carried out a large-scale CRISPR-based screen to assess the function of ∼17,000 lncRNAs in seven different human cell lines. A considerable number (∼500) of ...

CRISPRlnc: a manually curated database of validated sgRNAs for ...

The CRISPR/Cas9 system, as a revolutionary genome editing tool for all areas of molecular biology, provides new opportunities for research on ...

CRISPR Guide - Addgene

The gRNA is a short synthetic RNA composed of a scaffold sequence necessary for Cas-binding and a user-defined ∼20-nucleotide spacer that defines the genomic ...

CRISPR-Based Approaches for the High-Throughput ... - MDPI

Here, we review how various CRISPR-based strategies have been used to characterize lncRNAs with important functions in different biological contexts and how ...

Genomic Editing of Non-Coding RNA Genes with CRISPR/Cas9 ...

The application of the novel CRISPR/Cas9 technology in schizophrenia-associated ncRNAs will usher in a new perspective for schizophrenia ...

Platforms for Investigating LncRNA Functions - ScienceDirect

CRISPR tools can be employed to ablate lncRNA expression or function by direct mutagenesis of the DNA sequence. However, it must be noted that unlike protein ...

CRISPR Study Reveals Unexpected Roles of Non-Coding RNAs

In collaboration with the lab of Howard Chang, MD, PhD, of Stanford University, the team found that although about 5,000 different lncRNA genes ...

Targeting non-coding RNAs with the CRISPR/Cas9 system in ...

A most commonly used approach for gene functional study is knockdown by RNA inference (RNAi) which is mainly functional in the cytoplasm where ...

Genome-wide screening for functional long noncoding RNAs in ...

A team from Peking University used CRISPR gene editing to target splice sites of long non-coding RNAs, thereby knocking out their function. By ...