Generation of a Stable Cell Line Producing High|Titer Self ...

Generation of a stable cell line producing high-titer self-inactivating ...

To facilitate the generation of SIN lentivirus vector-producer cell lines, we have developed a novel conditional SIN (cSIN) lentivirus vector, which retains ...

Generation of a Stable Cell Line Producing High-Titer Self ...

We have developed a novel conditional SIN (cSIN) lentivirus vector, which retains its SIN properties in normal target cells yet can be produced at high titers.

Generation of a stable packaging cell line producing high-titer PPT ...

Generation of a stable packaging cell line producing high-titer PPT-deleted integration-deficient lentiviral vectors · Authors · Affiliations.

Generation of a stable packaging cell line producing high-titer PPT ...

Stable transfection of vector DNA to packaging cells is the second methodology described in this study for generating stable IDLV producing cell lines. We ...

Xu, K., Ma, H., McCown, T.J., Verma, I.M. & Kafri, T. Generation of a ...

Xu, K., Ma, H., McCown, T.J., Verma, I.M. & Kafri, T. Generation of a stable cell line producing high-titer self-inactivating lentiviral vectors. Mol. Ther. 3, ...

Generation of High-Titer Self-Inactivated γ-Retroviral Vector ...

High-titer retrovirus producer clones were also easily established from the 293Vec-RD114 and 293Vec-Ampho cells with three different SIN retroviral vectors ...

LentiPro26: novel stable cell lines for constitutive lentiviral vector ...

Herein, is described an alternative methodology to accelerate the establishment of LV producer cell lines presenting high titers, exclusively by ...

Generation of stable suspension producer cell lines for serum‐free ...

The derived monoclonal cell lines are functionally stable in continuous culture and produce an average infectious titer of up to 9.38 × 107 TU ...

Construction of stable packaging cell lines for clinical lentiviral ...

Here, we report a method that solves all these problems for generation of stable LV packaging and producer cell lines exemplified by the ...

Efficient construction of producer cell lines for a SIN lentiviral vector ...

Further refinement of our technique enabled the rapid generation of whole populations of stably transformed cells that produced similar titers. Finally, we ...

Generating stable cell lines with quantifiable protein production ...

However, generating recombinant protein–expressing cell lines is usually a lengthy process and the resulting protein expression levels are often inconsistent.

Establishment and Characterization of a Stable Producer Cell Line ...

While this method is efficient at small scales and has also proven to be scalable, the industry is exploring alternative processes due to the high cost of GMP ...

Scalable Lentiviral Vector Production Using Stable HEK293SF ...

The stable producer cell line, called clone 92, was derived by stable transfection from a packaging cell line with a plasmid encoding the transgene GFP. The ...

A quick and efficient method to generate mammalian stable cell ...

For that purpose, we developed a master cell line (MCL) containing an inducible alphavirus vector expressing GFP integrated into the genome. In ...

Virus Protocol - Generating Stable Cell Lines - Addgene

This protocol can be used to generate stable cell lines expressing a gene of interest from an integrated lentiviral vector.

Development of Stable Packaging and Producer Cell Lines for the ...

This article presents the development of HeLa cell-based packaging/producer cell lines up to their use for large-scale rAAV vector production.

Stable Cell Line Development Platform - Sino Biological

Stable cell lines express exogenous genes constitutively. Due to their high stability, they are suitable for recombinant protein and antibody production to ...

Development of a perfusion process for continuous lentivirus ...

The bioreactors were inoculated with a stable producer cell line encoding for a LV carrying a clinically relevant gene. LV production was ...

Generation of Cell Lines Capable of Producing High-titer Viral ...

These newly created cell lines can produce model clinical viruses and adeno-associated viruses at titers much higher than the parental cell lines.

Stable Cell Line Development Services - Sino Biological

Sino Biological stable cell line generation in CHO, HEK293 possess abundant stable cell line development experience for protein and antibody production.