Genome engineering using the CRISPR|Cas9 system

Genome engineering using the CRISPR-Cas9 system - Nature

Targeted nucleases are powerful tools for mediating genome alteration with high precision. The RNA-guided Cas9 nuclease from the microbial ...

Genome engineering using the CRISPR-Cas9 system - PubMed

Targeted nucleases are powerful tools for mediating genome alteration with high precision. The RNA-guided Cas9 nuclease from the microbial clustered ...

Genome engineering using the CRISPR-Cas9 system - PMC

Here we describe a set of tools for Cas9-mediated genome editing via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells.

What are genome editing and CRISPR-Cas9?: MedlinePlus Genetics

CRISPR-Cas9 was adapted from a naturally occurring genome editing system that bacteria use as an immune defense. When infected with viruses ...

The CRISPR tool kit for genome editing and beyond - Nature

Different CRISPR systems and their uses in genome editing. The evolutionary arms race between prokaryotes and environmental mobile genetic ...

Genome Editing with CRISPR-Cas9 - YouTube

This animation depicts the CRISPR-Cas9 method for genome editing – a powerful new technology with many applications in biomedical research, ...

Genome Engineering Using CRISPR-Cas9 System | SpringerLink

The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas9 system is an adaptive immune system that exists in a variety of ...

Genome Engineering via CRISPR-Cas9 System - ScienceDirect.com

CRISPR-Cas system is a key genome editing technology which is simple, cost-effective, specific and user-friendly.

Questions and Answers about CRISPR | Broad Institute

... system that forms the basis for CRISPR-Cas9 genome editing technology. In ... Q: What other scientific uses might CRISPR have beyond genome editing? A ...

Genome Engineering with CRISPR-Cas9: Birth of a Breakthrough ...

Doudna and her colleagues developed the CRISPR-Cas9 gene expression system that, when introduced into animal cells, makes site-specific changes to intact ...

Multiplex Genome Engineering Using CRISPR/Cas Systems - Science

We engineered two different type II CRISPR/Cas systems and demonstrate that Cas9 nucleases can be directed by short RNAs to induce precise cleavage at ...

How To Use CRISPR: Your Guide to Successful Genome Engineering

The CRISPR-Cas9 system has revolutionized the field of genome engineering with limitless applications in disease therapeutics, drug discovery, agriculture, ...

Efficient genome engineering in human pluripotent stem cells using ...

Here, using a distinct CRISPR-Cas system from Neisseria meningitidis, we demonstrate efficient targeting of an endogenous gene in three hPSC lines using HDR.

Multigene Editing in the Escherichia coli Genome via the CRISPR ...

Therefore, we developed a CRISPR-Cas9 system-based continual genome editing strategy, including gene insertions and knockouts of both single and multiple (up to ...

Zebrafish Genome Engineering Using the CRISPR–Cas9 System

CRISPR–Cas9 is an RNA-directed endonuclease that can generate double-strand breaks (DSBs) in the zebrafish genome.

The new frontier of genome engineering with CRISPR-Cas9 - Science

The advent of facile genome engineering using the bacterial RNA-guided CRISPR-Cas9 system in animals and plants is transforming biology.

Genome engineering in Saccharomyces cerevisiae using CRISPR ...

Abstract. Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated (Cas) systems in bacteria and archaea use RNA-guided nuc.

[PDF] Genome engineering using the CRISPR-Cas9 system

A set of tools for Cas9-mediated genome editing via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, ...

Genome engineering using the CRISPR-Cas9 system - DSpace@MIT

Here we describe a set of tools for Cas9-mediated genome editing via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells.

Genome engineering using CRISPR-Cas9 system. | Broad Institute

The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas9 system is an adaptive immune system that exists in a variety of microbes.