Harnessing DSB repair to promote efficient homology|dependent and

Harnessing DSB repair to promote efficient homology-dependent and

Prime editing recently emerged as a next-generation approach for precise genome editing. Here we exploit DNA double-strand break (DSB) ...

Harnessing DSB repair to promote efficient homology-dependent and

Download Citation | Harnessing DSB repair to promote efficient homology-dependent and -independent prime editing | Prime editing recently emerged as a ...

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Harnessing DSB repair to promote efficient homology-dependent ...

Prime editing recently emerged as a next-generation approach for precise genome editing. Here we exploit DNA double-strand break (DSB) repair to develop two ...

Harnessing DSB repair to promote efficient homology-dependent ...

AbstractPrime editing recently emerged as a next-generation approach for precise genome editing. Here we exploit DNA double-strand break (DSB) repair to ...

[PDF] Harnessing DSB repair to promote efficient homology ...

A nuclease-based prime editor that leverages DNA repair pathways for targeted genomic insertions is designed and developed, which expands the current prime ...

The biological principles and advanced applications of DSB repair ...

The discussion of DSB repair covers the most frequently used homologous recombination (HR) and nonhomologous end joining (NHEJ) strategies.

Targeting double-strand break indel byproducts with secondary ...

Programmable double-strand DNA breaks (DSBs) can be harnessed for precision genome editing through manipulation of the homology-directed ...

Harnessing accurate non-homologous end joining for efficient ...

In mammalian cells, Cas9-induced DSBs are repaired largely by non-homologous end joining (NHEJ) and partly by homology-directed repair (HDR) ...

Altered DNA repair pathway engagement by engineered ... - PNAS

Consequently, vCas9 enables efficient precise editing through HDR or MMEJ while suppressing indels caused by NHEJ in dividing and nondividing cells. These ...

CRISPR-Cas9-mediated homology-directed repair for precise gene ...

Thus, strategies for enhancing HDR efficiency can be divided into four main categories: inhibition of the NHEJ DNA repair pathway, activation of ...

Methods Favoring Homology-Directed Repair Choice in Response ...

In this review, we summarize the DSB repair pathways, the mechanisms involved in choice selection based on DNA resection, and make progress in the research.

Harnessing RNA-based DNA repair pathways for targeted ... - bioRxiv

However, a major challenge in the gene-editing field is the heterogeneous nature of repair events that impact the efficiency, fidelity, and ...

CRISP_SCIENCE on X: ""Harnessing DSB repair to promote ... - X.com

"Harnessing DSB repair to promote efficient homology-dependent and -independent prime editing" Peterka M, Akrap N, Li S, Wimberger S [.

DNA Repair Pathway Choices in CRISPR-Cas9-Mediated Genome ...

Thus, the accuracy and efficiency of genome editing are influenced by the cellular DSB repair pathways. DSBs are themselves highly genotoxic lesions and as such ...

Methods for Enhancing Clustered Regularly Interspaced Short ...

Methods for DSB repair include the NHEJ and HDR pathway. The NHEJ pathway creates accurate deletions and insertions. The HDR pathway uses ...

Improving integration efficiency and precision of CRISPR/Cas9 ...

End joining repair can result in imprecise DNA insertions and deletions that may be used to disrupt gene function. At the same time, HDR enables ...

CRISPR 101: Homology Directed Repair - Addgene Blog

HDR uses homologous sequence as a template for repair and is thus the more accurate mechanism for DSB repair. The process is generally error- ...

bioRxiv on X: "Harnessing DSB repair to promote efficient homology ...

Harnessing DSB repair to promote efficient homology-dependent and -independent prime editing https://t.co/qygPxpPsZx #bioRxiv.

Harnessing DNA Double-Strand Break Repair for Cancer Treatment

Therefore, DSB repair represents a potent and targetable vulnerability in cancer cells. In healthy somatic cells two-ended DSBs are mainly ...