High|speed volumetric two|photon fluorescence imaging of ...

High-speed volumetric two-photon fluorescence imaging of ... - Nature

The axially elongated excitation volume of Bessel foci produced two-photon fluorescence signal that was proportional to vessel size, which ...

High-speed volumetric two-photon fluorescence imaging of ...

Title: High-speed volumetric two-photon fluorescence imaging of neurovascular dynamics. Journal Article · Thu Nov 26 00:00:00 EST 2020 · Nature ...

High-speed volumetric two-photon fluorescence imaging of ...

Understanding the structure and function of vasculature in the brain requires us to monitor distributed hemodynamics at high spatial and temporal resolution ...

High-speed volumetric two-photon fluorescence imaging of ...

... speed. Here, we applied two-photon microscopy with an axially extended Bessel focus to fast volumetric imaging of vasculature in the awake brain ...

[PDF] High-speed volumetric two-photon fluorescence imaging of ...

High-throughput two-photon microscopy with an axially-extended Bessel focus is used to measure vessel size and blood flow down to capillary ...

Volumetric two-photon fluorescence imaging of live neurons using a ...

Volumetric two-photon fluorescence imaging of live ... The SLM refresh rate limits the imaging speed ... high dynamic range imaging with linear fluorescence.

Ultrafast two-photon fluorescence imaging of cerebral blood ... - PNAS

J. L. Fan et al., High-speed volumetric two-photon fluorescence imaging of neurovascular dynamics. Nat. Commun. 11, 6020 (2020). Crossref.

High-speed volumetric two-photon fluorescence imaging of ...

Currently, a volumetric vasculature imaging method with sub-capillary spatial resolution and blood flow-resolving speed is lacking. Here, using two-photon laser ...

Dual GRIN lens two-photon endoscopy for high-speed volumetric ...

One is to capture flow speed of fluorescent microspheres that imitates blood flow. The other one is to achieve in vivo functional imaging of neuronal activity ...

High-throughput two-photon volumetric brain imaging in ... - bioRxiv

Lateral and axial resolution measured with fluorescent beads were 0.97–0.99 μm and 80–90 μm across the FOV (Supplementary Fig. 5). The lateral ...

Two-Photon Fluorescence Imaging - PubMed

... high-speed, high-resolution, large-volume, long-term imaging in freely behaving animals. Keywords: Miniature two-photon microscope; Pan-neuronal imaging ...

High-speed two-color scanning volumetric laser-induced fluorescence

Many problems in fluid mechanics require single-shot 3D measurements of fluid flows, but are limited by available techniques.

High-throughput synapse-resolving two-photon fluorescence ... - eLife

Improved two-photon fluorescence microendoscopy enables volumetric imaging of synapses and neurons in deep brain structures in vivo.

Dual-objective two-photon microscope for volumetric imaging of ...

Three-photon microscopy, with its high-order nonlinear excitation, presents a promising avenue for extending the imaging depth in scattering ...

Rapid, high-contrast, and steady volumetric imaging with Bessel ...

PDF | Significance Two-photon fluorescence microscopy (TPFM) excited by Gaussian beams requires axial tomographic scanning for ...

Two-photon excitation microscopy - Wikipedia

Two-photon excitation microscopy (TPEF or 2PEF) is a fluorescence imaging technique that is particularly well-suited to image scattering living tissue of up ...

Dual-resonant scanning multiphoton microscope with ultrasound ...

... high-speed volumetric imaging. In the ... High-speed, two-photon scanning microscope. ... Kilohertz two-photon fluorescence microscopy imaging ...

Volumetric Two-photon Imaging of Neurons Using Stereoscopy ...

Two-photon laser scanning microscopy of calcium dynamics using fluorescent indicators is a widely used imaging method for large-scale recording of neural ...

Two-photon scanned light sheet fluorescence microscopy with ...

... imaging by recording the rapid dynamics of physiological processes. 1. Introduction. Techniques to capture deep volumetric images at high speed ...

Scanning two photon fluorescence microscopy with extended depth ...

... Volume 188, 2018, pp. 135-140. Francesco Crisafi, …, Dario Polli. Faster fluorescence microscopy: advances in high speed biological imaging. Current Opinion in ...