Interrogating lncRNA functions via CRISPR/Cas systems

Interrogating lncRNA functions via CRISPR/Cas systems - PMC - NCBI

In this review, we summarize how the CRISPR/Cas technology provides new tools to investigate the roles and therapeutic implications of lncRNAs.

Interrogating lncRNA functions via CRISPR/Cas systems - PubMed

Long noncoding RNAs (lncRNAs) are an increasing focus of investigation due to their implications in diverse biological processes and disease.

Interrogating lncRNA functions via CRISPR/Cas systems: RNA Biology

In this review, we summarize how the CRISPR/Cas technology provides new tools to investigate the roles and therapeutic implications of lncRNAs.

Interrogating lncRNA functions via CRISPR/Cas systems

The CRISPR/Cas system, an innovative technology that has emerged over the last decade, can be utilized to further understand lncRNA function. The system targets ...

Techniques for investigating lncRNA transcript functions in ... - Nature

The CRISPR/Cas system has become popular for use in high-throughput forward genetic screens, transcription interference (CRISPRi), and gene ...

Genome-scale pan-cancer interrogation of lncRNA dependencies ...

Here we developed genome-scale lncRNA transcriptome screening using Cas13d/CasRx. We show that RNA targeting overcomes limitations inherent to other screening ...

CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

Scientists have determined the function of many protein-coding genes thanks to systems like CRISPR/Cas9 that allow them to manipulate full genes ...

CRISPR/Cas-based screening of long non-coding RNAs (lncRNAs ...

We also established a CRISPR/Cas9 gene deletion system in this NF-κB reporter line, enabling us to screen for genes that regulate NF-κB signaling. Our deletion- ...

Interrogating genome function using CRISPR tools: a narrative review

However, the targets of noncoding regulatory elements and the precise function of ncRNAs are, for the most part, still unknown. CRISPR/Cas9 system based genetic ...

CRISPR-based technology to silence the expression of IncRNAs

Recent efforts to identify functional lncRNAs have relied on CRISPR technology, which can exquisitely target CRISPR-associated (Cas) proteins to ...

CRISPR links to long noncoding RNA function in mice: A practical ...

Here, CRISPR genome editing components are updated for elucidating lncRNA function in mice. Various strategies are highlighted for understanding the function ...

Emerging Functions of lncRNA Loci beyond the Transcript Itself - MDPI

In our experience, initial experimental strategies including deletion of promoter and exon sequences by CRISPR-Cas9 are the most reliable, ...

innovative CRISPR approaches for functional studies of long non ...

Using CRISPR/Cas9 system, silencing signals could be inserted in critical key regions along the lncRNA locus, thereby inhibiting its expression.

Designing libraries for pooled CRISPR functional screens of long ...

Human and other genomes encode tens of thousands of long noncoding RNAs (lncRNAs), the vast majority of which remain uncharacterised.

Application of CRISPR-Cas9 for Long Noncoding RNA Genes in ...

Growing evidence shows that LncRNAs can be targeted by the CRISPR-Cas9 system used for evaluating its function. Thus, the CRISPR-Cas9 systems provide a novel ...

(PDF) Techniques for investigating lncRNA transcript functions in ...

... CRISPR/Cas systems and applications to experimental neurodeve- ... interrogation of gene function in the mammalian brain using CRISPR-Cas9. Nat.

CRISPR links to long noncoding RNA function in mice: A practical ...

Next generation sequencing has uncovered a trove of short noncoding RNAs (e.g., microRNAs) and long noncoding RNAs (lncRNAs) that act as molecular rheostats ...

Challenges of CRISPR/Cas9 applications for long non-coding RNA ...

The CRISPR/Cas9 system provides a revolutionary genome editing tool for all areas of molecular biology. In long non-coding RNA (lncRNA) research, the Cas9 ...

Profiling Therapeutically Actionable Long Non-coding RNAs Using ...

Such “onco-lncRNAs” have resisted traditional RNAi methods, but CRISPR-Cas9 genome editing now promises functional screens at high throughput ...

Modulating the expression of long non‐coding RNAs for functional ...

Double Cas9 excision of DNA sequences that flank lncRNAs are more likely to inactivate lncRNA gene function and has been used to delete up to ...