Isolation and culture of colon cancer stem cells.

Isolation and culture of colon cancer stem cells - PubMed

Cancer stem cells (CSCs) resemble normal stem cells in several ways. Both cell types are self-renewing and when they divide, one of the daughter cells ...

Isolation and Culture of Colon Cancer Stem Cells - ScienceDirect.com

There are essentially three different methods to isolate CSCs: establishment culture, the MACS (magnetic cell sorting) technology, and the FACS (fluorescence‐ ...

Isolation and Characterization of Human Colon Adenocarcinoma ...

For colon cancer, the most actively studied markers of the cancer stem cell are: CD133+ [12,13], CD44+ [14,15], EpCAM [16], CD29, CD24 [17], CD166 [18], ...

The requirement for freshly isolated human colorectal cancer (CRC ...

Isolation of colorectal cancer (CRC) cell populations enriched for cancer stem cells (CSCs) may facilitate target identification.

Isolation and Culture of Colon Cancer Stem Cells

Isolation and Culture of Colon Cancer Stem Cells. 16 October 2023; [email protected]. Related Items. Sparse inference of the human ...

Isolation and Propagation of Colon Cancer Stem Cells - SpringerLink

The design of tissue culture conditions that faithfully reproduce the characteristics of cells in their native environment remains one of the main ...

Cancer stem cells from colorectal cancer-derived cell lines - PNAS

There is strong evidence that cell lines represent the tumors from which they were originally isolated (7) and can develop structures similar to those found in ...

Isolation and Characterization of Intestinal Stem Cells Based on ...

We compared different combinations of surface markers among ISCs isolated based on expression of Lgr5–green fluorescent protein. We developed a culture protocol ...

Isolation and Characterization of Two Novel Colorectal Cancer Cell ...

Cancer stem cells (CSC) are crucial mediators of cancer relapse. Here, we isolated two primary human colorectal cancer cell lines derived from a rectal ...

Colorectal Cancer Stem Cells: From the Crypt to the Clinic

By exploiting a combination of genetic, epigenetic, and microenvironmental factors, colorectal CSCs metastasize, resist chemotherapy, and ...

Expansion of CD133+ colon cancer cultures retaining stem cell ...

Our data suggest that ex vivo-expanded colon CSCs isolated from clinical specimens can be maintained in culture enabling the identification of CSC cell surface ...

Isolation and characterization of chemo-resistant stem cells | SCCAA

These treatment options lead to systemic toxicity, acquired tumor resistance and the emergence of drug-resistant stem cells. A colonic ...

Isolation and Culture of Colon Cancer Stem Cells | Request PDF

CSC isolation and identification methods include fluorescence-activated cell sorting (FACS), magnetic-activated cell sorting (MACS) and serum-free medium (SFM) ...

Suspension culture strategies to enrich colon cancer stem cells

Increasing evidence suggests that targeting and selective killing of CSCs to prevent tumor metastasis, recurrence and drug resistance may ...

CD66c is a novel marker for colorectal cancer stem cell isolation ...

The isolation and characterization of tumorigenic colon cancer cells play major roles in the development of new diagnostic and therapeutic ...

Colorectal Cancer Stem Cells: An Overview of Evolving Methods ...

According to the stem cell model, most tumors, including colorectal cancer (CRC), contain a small population of cancer stem cells (CSCs) that are deeply ...

Isolation and Propagation of Colon Cancer Stem Cells

Techniques. Cell And Tissue Culture. Co-culture. Tissue Digestion. Dissection ; Reagents · Antibodies. Protein. Experimental Models. CCD-18Co; Colon; Human (Homo ...

Colon Cancer Stem Cells: Promise of Targeted Therapy

First developed for hematologic disorders, the concept of cancer stem cells (CSCs) was expanded to solid tumors, including colorectal cancer (CRC).

Cancer Stem Cell-Based Models of Colorectal ... - Oxford Academic

Colorectal cancer stem cell (CSC)-enriched cultures were obtained efficiently from primary tumor specimens by optimizing a CSC-isolation protocol. Based on.

A Stochastic Model for Cancer Stem Cell Origin in Metastatic Colon ...

Tumorigenicity and multilineage commitment of clonally derived metastatic colon tumor cells. A, CEA secretion in vitro in long-term cultures and ...

Isolation and culture of colon cancer stem cells.

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