Isolation and detection of DNA|protein crosslinks in mammalian cells

Isolation and detection of DNA-protein crosslinks in mammalian cells

DNA-protein crosslinks (DPCs) are toxic DNA lesions wherein a protein is covalently attached to DNA. If not rapidly repaired, ...

Isolation and detection of DNA–protein crosslinks in mammalian cells

Here, we critically analyse the latest techniques for DPC isolation and the benefits and drawbacks of each.

Isolation and detection of DNA-protein crosslinks in mammalian cells

PDF | DNA–protein crosslinks (DPCs) are toxic DNA lesions wherein a protein is covalently attached to DNA. If not rapidly repaired, ...

Isolation and Immunodetection of Enzymatic DNA-Protein Crosslinks ...

The method involves isolating genomic DNA and DPCs from cells and binding them to nitrocellulose membrane with a vacuum slot blot manifold.

Isolation and detection of DNA-protein crosslinks in mammalian cells.

DNA-protein crosslinks (DPCs) are toxic DNA lesions wherein a protein is covalently attached to DNA. If not rapidly repaired, ...

Identification of Mammalian Proteins Cross-linked to DNA by ...

Proteins identified in IR-induced DNA-protein cross-links Note: most peptides were detected in multiple experiments, and modified peptides were also detected ( ...

Kristijan Ramadan Laboratory on X: "The lab is thrilled to see our ...

... isolation and detection of DNA-protein crosslinks: https ... Isolation and detection of DNA–protein crosslinks in mammalian cells.

Isolation and detection of DNA–protein crosslinks in mammalian cells

Abstract DNA–protein crosslinks (DPCs) are toxic DNA lesions wherein a protein is covalently attached to DNA. If not rapidly repaired, DPCs create obstacles ...

Isolation and detection of DNA–protein crosslinks in mammalian cells

Isolation and detection of DNA–protein crosslinks in mammalian cells · Nucleic Acids Research, December 2023 · 10.1093/nar/gkad1178 · 38084926.

Quantification of Intracellular DNA–Protein Cross-Links with N7 ...

(25,41,44,46−48) MS was employed to detect DPCs between MdG and histone proteins by taking advantage of their reversibility. (10) Briefly, the ...

The ARK Assay Is a Sensitive and Versatile Method for the Global ...

DNA-protein crosslinks (DPCs) are a form of DNA damage generated when a protein moiety is covalently conjugated to cellular DNA. The exceedingly ...

Detection of DNA–protein crosslinks (DPCs) by novel direct ...

Proteins are covalently trapped on DNA to form DNA–protein crosslinks (DPCs) when cells are exposed to DNA-damaging agents. DPCs interfere with many aspects of ...

Isolation and Immunodetection of Enzymatic DNA–Protein ...

The method involves isolating genomic DNA and DPCs from cells and binding them to nitrocellulose membrane with a vacuum slot blot manifold.

SPRTN patient variants cause global-genome DNA-protein crosslink ...

Here, we develop a technique for the Purification of x-linked Proteins (PxP), which allows identification and tracking of diverse DPCs in ...

Quantitative Detection of DNA-Protein Crosslinks and Their Post ...

DNA-protein crosslinks (DPCs) are frequent, ubiquitous, and deleterious DNA lesions, which arise from endogenous DNA damage, enzyme ( ...

Enzymatic Processing of DNA–Protein Crosslinks - MDPI

DPCs are formed through a variety of mechanisms and can significantly impede essential cellular processes such as transcription and replication. For this reason ...

Cdc48/p97 segregase: Spotlight on DNA-protein crosslinks

Isolation and detection of DNA-protein crosslinks in mammalian cells. Nucleic ... Nucleotide excision repair eliminates unique DNA-protein cross-links from ...

DNA–Protein Cross-Links: Formation, Structural Identities, and ...

DNA–protein cross-links (DPCs) form when cellular proteins become covalently trapped on DNA strands upon exposure to various endogenous, ...

Endogenous aldehyde-induced DNA–protein crosslinks are ... - Nature

b, Chromatin fractions of WT and ΔCSB HeLa cells after formaldehyde treatment or UV irradiation were subjected to immunoprecipitation with anti- ...

Purification of DNA repair protein complexes from mammalian cells

The crosslinked nuclei should be sonicated over a time-course to identify optimal condition for protein extraction. We monitor the extraction of ...