- Isolation and detection of DNA|protein crosslinks in mammalian cells🔍
- Isolation and detection of DNA–protein crosslinks in mammalian cells🔍
- Isolation and Immunodetection of Enzymatic DNA|Protein Crosslinks ...🔍
- Isolation and detection of DNA|protein crosslinks in mammalian cells.🔍
- Identification of Mammalian Proteins Cross|linked to DNA by ...🔍
- Kristijan Ramadan Laboratory on X🔍
- Detection of DNA–protein crosslinks 🔍
- The ARK Assay Is a Sensitive and Versatile Method for the Global ...🔍
Isolation and detection of DNA–protein crosslinks in mammalian cells
Isolation and detection of DNA-protein crosslinks in mammalian cells
DNA-protein crosslinks (DPCs) are toxic DNA lesions wherein a protein is covalently attached to DNA. If not rapidly repaired, ...
Isolation and detection of DNA–protein crosslinks in mammalian cells
Here, we critically analyse the latest techniques for DPC isolation and the benefits and drawbacks of each.
Isolation and Immunodetection of Enzymatic DNA-Protein Crosslinks ...
The method involves isolating genomic DNA and DPCs from cells and binding them to nitrocellulose membrane with a vacuum slot blot manifold.
Isolation and detection of DNA-protein crosslinks in mammalian cells
PDF | DNA–protein crosslinks (DPCs) are toxic DNA lesions wherein a protein is covalently attached to DNA. If not rapidly repaired, ...
Isolation and detection of DNA-protein crosslinks in mammalian cells.
DNA-protein crosslinks (DPCs) are toxic DNA lesions wherein a protein is covalently attached to DNA. If not rapidly repaired, ...
Identification of Mammalian Proteins Cross-linked to DNA by ...
Proteins identified in IR-induced DNA-protein cross-links Note: most peptides were detected in multiple experiments, and modified peptides were also detected ( ...
Kristijan Ramadan Laboratory on X: "The lab is thrilled to see our ...
... isolation and detection of DNA-protein crosslinks: https ... Isolation and detection of DNA–protein crosslinks in mammalian cells.
Isolation and detection of DNA–protein crosslinks in mammalian cells
Isolation and detection of DNA–protein crosslinks in mammalian cells · Nucleic Acids Research, December 2023 · 10.1093/nar/gkad1178 · 38084926.
Isolation and detection of DNA–protein crosslinks in mammalian cells
Abstract DNA–protein crosslinks (DPCs) are toxic DNA lesions wherein a protein is covalently attached to DNA. If not rapidly repaired, DPCs create obstacles ...
Detection of DNA–protein crosslinks (DPCs) by novel direct ...
Proteins are covalently trapped on DNA to form DNA–protein crosslinks (DPCs) when cells are exposed to DNA-damaging agents. DPCs interfere with many aspects of ...
The ARK Assay Is a Sensitive and Versatile Method for the Global ...
DNA-protein crosslinks (DPCs) are a form of DNA damage generated when a protein moiety is covalently conjugated to cellular DNA. The exceedingly ...
Quantification of Intracellular DNA–Protein Cross-Links with N7 ...
Isolation and Detection of DNA–Protein Crosslinks in Mammalian Cells. Nucleic Acids Res. 2024, 52, 525– 547, DOI: 10.1093/nar/gkad1178.
Quantitative Detection of DNA-Protein Crosslinks and Their Post ...
DNA-protein crosslinks (DPCs) are frequent, ubiquitous, and deleterious DNA lesions, which arise from endogenous DNA damage, enzyme ( ...
Isolation and Immunodetection of Enzymatic DNA–Protein ...
The method involves isolating genomic DNA and DPCs from cells and binding them to nitrocellulose membrane with a vacuum slot blot manifold.
Transcription-coupled repair of DNA–protein cross-links depends on ...
Covalent DNA–protein cross-links (DPCs) are toxic DNA lesions that block replication and require repair by multiple pathways.
A method for the isolation of covalent DNA–protein crosslinks ...
This article describes a novel method for the isolation of DPCs from mammalian cells that uses chaotropic agents to isolate genomic DNA and ...
Enzymatic Processing of DNA–Protein Crosslinks - MDPI
DPCs are formed through a variety of mechanisms and can significantly impede essential cellular processes such as transcription and replication. For this reason ...
SPRTN patient variants cause global-genome DNA-protein crosslink ...
Here, we develop a technique for the Purification of x-linked Proteins (PxP), which allows identification and tracking of diverse DPCs in ...
A simple, sensitive assay to detect DNA-protein crosslinks in intact ...
The K--SDS assay was also successfully utilized to determine DNA--protein crosslinks in rat and mouse white blood cells exposed in vivo to chromate and ...
Purification of DNA repair protein complexes from mammalian cells
The crosslinked nuclei should be sonicated over a time-course to identify optimal condition for protein extraction. We monitor the extraction of ...