Isotope Labeling in Metabolomics and Fluxomics

Isotope labeling experiments in metabolomics and fluxomics - PubMed

Metabolomics, the study of all the small molecules in and outside a cell and fluxomics, comprising all conversion rates in a cell, are increasingly used in ...

Isotope labeling experiments in metabolomics and fluxomics

Such fluxomics experiments require the measurement of intracellular and extracellular metabolites and cellular polymers. The quantification of ...

Stable isotope-labeling studies in metabolomics: new insights into ...

The natural abundance of stable isotopes is often exploited in label-free metabolomics studies to assist metabolite identification, in combination with accurate ...

Global stable-isotope tracing metabolomics reveals system-wide ...

Stable-isotope tracing metabolomics allows to unravel metabolic activity quantitatively by measuring the isotopically labeled metabolites, but ...

Isotope labeling experiments in metabolomics and fluxomics

Each molecule with n carbon atoms has n + 1 carbon mass isotopomers. for the elucidation of pathways and in flux analysis.14,15 Stable isotopes are completely ...

Fluxomics - New Metabolomics Approaches to Monitor Metabolic ...

Another practical example of isotope labelling is to identify isotopomers (one of the different labeling states in which a particular metabolite ...

Stable isotope-resolved metabolomics based on mass spectrometry

There are two isotope labeling methods for SIRM [15], and their processes are shown in Fig. 1. One is metabolic labeling, which introduces targeted substrates ...

Isotope Labeling in Metabolomics and Fluxomics, Charles Evans

Understand the difference between normal metabolomics and a flux experiment; how to perform an experiment using a stable isotope.

Isotope labeling experiments in metabolomics and fluxomics

For both fluxomics and metabolomics the application of isotopes became almost indispensable, and their use in these techniques is discussed, ...

Applications of stable isotope‐based metabolomics and fluxomics ...

A special case of INST-MFA, termed kinetic flux profiling (KFP), has also been applied to determine metabolic fluxes from transient isotope labeling data. In ...

Stable Isotope Labeled Metabolomics Improves Identification of ...

Keywords: carbon-13 ▫ fluxomics ▫ metabolic pathway ▫ metabolite identification ▫ metabolomics ▫ stable isotope labelling ... isotope labeling with metabolomics ...

SIMPEL: using stable isotopes to elucidate dynamics of context ...

In the first description, a dual-isotope labeling experiment is paired with SIMPEL and isotopically nonstationary metabolic flux analysis (INST- ...

Tracing metabolic flux through time and space with isotope labeling ...

Isotopic tracers (i.e. stable isotopes namely 13C, 15N, 2H, 18O, etc. or radioisotopes such as 14C or 3H) are the primary tools used by ...

Isotope labeling experiments in metabolomics and fluxomics

Mass Spectrometry (MS) is particularly suitable to detect stable isotopes (Klein and Heinzle, 2012) , because the relative abundance of isotopes within a ...

Parallel isotope differential modeling for instationary 13C fluxomics ...

Here, we present a parallelized method to model instationary 13C labeling data. The elementary metabolite unit (EMU) framework is reorganized to ...

Strategies for Extending Metabolomics Studies with Stable Isotope ...

The session highlighted the utility of stable isotope labelling in generating reference standards for metabolite identification, absolute ...

Applications of Stable Isotope Labeling in Metabolic Flux Analysis

Stable isotope labeling is a sophisticated technique, prominently recognized in the realm of metabolic flux analysis (MFA), that allows researchers to map the ...

Metabolome-Wide Analysis of Stable Isotope Labeling—Is It Worth ...

Because isotopic labeling patterns are a direct consequence of metabolic fluxes, changes in these patterns indicate metabolic flux changes (Sauer, 2006).

Fluxomics – University of Copenhagen

Additionally, isotopic labeling experiments help us understand which substrates a given metabolite is derived from and can provide crucial information about ...

Complex Cells Metabolism Measurement by Stable Isotope Tracing

Isotope Labeling in Metabolomics and Fluxomics, Charles Evans. MetabolomicsMI ; SILAC | Stable isotope labeling by amino acids in cell culture | ...