Isotope Tracer Analysis and Metabolic Flux Analysis

Measurement of metabolic fluxes using stable isotope tracers in ...

Metabolic flux analysis using stable isotope labeled substrates allows for the tracing of carbon, nitrogen, and hydrogen atoms through metabolic pathways.

Evaluation of 13C isotopic tracers for metabolic flux analysis in ...

13C metabolic flux analysis (MFA) is the most comprehensive means of characterizing cellular metabolic states. Uniquely labeled isotopic tracers enable more ...

Tracing metabolic flux in vivo: basic model structures of tracer ...

Over the past century, stable, non-radioactive isotope tracers have been widely used to provide critical information on the dynamics of specific ...

Isotope Tracer Analysis and Metabolic Flux Analysis

Both are powerful technique that can be integrated with conventional metabolomics to provide mechanistic insights into underlying metabolic changes.

A guide to 13 C metabolic flux analysis for the cancer biologist - Nature

The selection of an isotopic tracer (or multiple tracers) is one of the most important considerations when designing 13C-MFA studies, since this ...

Adapting isotopic tracer and metabolic flux analysis approaches to ...

Here we review recent studies that have developed isotopic tracer approaches to overcome the challenge of uniform metabolite labeling and provide quantitative ...

Metabolic Flux Analysis and In Vivo Isotope Tracing

Metabolic Flux Analysis and In Vivo Isotope Tracing ... Metabolic flux is the dynamic process of tracking the changes in metabolite levels throughout the entire ...

Metabolic Flux – Cambridge Isotope Laboratories, Inc.

Isotope tracers are essential tools for monitoring metabolic pathway activity, i.e. flux ... A guide to metabolic flux analysis in metabolic engineering ...

Tracing metabolic flux through time and space with isotope labeling ...

Dynamic labeling experiments can be used to cluster metabolites based on their turnover rate ... MS/MS analysis of metabolite extracts.

Basics of metabolite profiling and metabolic flux analysis

Isotopic tracing to study the activity of metabolic pathways. • Consider tracer uptake (e.g., glucose uptake) before performing the tracing.

Metabolic flux analysis - Wikipedia

Metabolic flux analysis (MFA) is an experimental fluxomics technique used to examine production and consumption rates of metabolites in a biological system.

Applications of Stable Isotope Labeling in Metabolic Flux Analysis

Stable isotope labeling is a sophisticated technique, prominently recognized in the realm of metabolic flux analysis (MFA), that allows researchers to map the ...

FluxFix: automatic isotopologue normalization for metabolic tracer ...

Isotopic tracer analysis by mass spectrometry is a core technique for the study of metabolism. Isotopically labeled atoms from substrates, ...

Metabolic flux analysis: a comprehensive review on sample ...

In the last decade, the application of stable isotopes became extremely important to determine and integrate in vivo measurements of metabolic ...

Isotopically nonstationary metabolic flux analysis of plants: recent ...

By introducing stable isotopic tracers and employing mathematical models, MFA can quantify the rates of metabolic reactions through biochemical ...

One‐shot 13C15N‐metabolic flux analysis for simultaneous ...

Recently, multiple stable isotopic tracers have been used to measure C and N enrichments and to derive insights into N assimilation in ...

Metabolic Flux Analysis—Linking Isotope Labeling and ... - MDPI

MFA uses stable isotope labeled tracers to reveal information related to the fluxes. The conceptual idea of MFA is that in tracer experiments ...

Stable Isotope Tracers for Metabolic Pathway Analysis

Stable isotope tracing allows a metabolic substrate to be followed through downstream biochemical reactions, thereby providing unparalleled insights into ...

Measuring Metabolic Flux - The Animation Lab

Metabolic flux can be inferred through the use of isotopic tracers along ... (Metabolic Flux Analysis) is a gold standard for accurate and precise flux information ...

iMS2Flux– a high–throughput processing tool for stable isotope ...

Metabolic flux analysis has become an established method in systems biology and functional genomics. The most common approach for ...