MHC Genotyping via Deep Sequencing

We have developed a MiSeq sequence-based genotyping assay for Mauritian cynomolgus macaques using a Fluidigm panel of exon 2 primers for the MHC class I and ...

Deep Sequencing of MHC-Adapted Viral Lines Reveals Complex ...

Instead, we found a high number of low frequency variants, possibly contributing to host genotype-specific adaptation through cryptic mechanisms ...

Rapid Identification of Major Histocompatibility Complex Class I ...

We tested a well-characterized MHC deep sequencing pipeline, developed for macaques (Karl et al. ... MHC genotyping of non-model organisms using next-generation ...

A new hybrid approach for MHC genotyping: high-throughput NGS ...

Our sequencing project was part of the MinION Access Programme (MAP) launched by Oxford Nanopore. MinION sequencing is rather inexpensive and ...

High-throughput, high-fidelity HLA genotyping with deep sequencing

Here we demonstrate a high-resolution, and cost-effective methodology to type HLA genes by sequencing, which combines the advantage of long-range amplification.

MHC genotyping of non-model organisms using next-generation ...

Next-generation sequencing (NGS) technologies are likely to replace traditional genotyping methods to a great extent in the near future but ...

Testing genotyping strategies for ultra-deep sequencing of a co ...

Using Illumina sequencing, Biedrzycka et al. [15] tested four genotyping approaches for characterizing MHC diversity in a passerine bird with the maximum number ...

Deep Sequencing of MHC-Adapted Viral Lines Reveals Complex ...

Here, we deep sequence these viral lines in an attempt to discover the genetic basis of FVC adaptation. The principal prediction for genotype- ...

Testing genotyping strategies for ultra‐deep sequencing of a co ...

Characterization of highly duplicated genes, such as genes of the major histocompatibility complex (MHC), where multiple loci often ...

MHC genotyping from rhesus macaque exome sequences - bioRxiv

MHC class I and class II genotyping by amplicon deep sequencing. Genomic DNA was isolated from 250μL of whole blood using a Maxwell® 48 LEV ...

Deep sequencing of the MHC region in the Chinese population ...

The performance of our reference panel was better than that of all the other panels when evaluating using the same genotype array and a ...

Benchmark of tools for in silico prediction of MHC class I and class II ...

HLAscan: genotyping of the HLA region using next-generation sequencing data. BMC Bioinformatics. 2017;18:1–11. Article Google Scholar. Lee H ...

Ultra‐deep Illumina sequencing accurately identifies MHC class IIb ...

We provide a simple, accurate and repeatable method for genotyping multigene families, evaluating our method via analyses of 209 b of MHC class ...

MHC genotyping from rhesus macaque exome sequences - bioRxiv

... MHC. 22 genotypes. In contrast, conventional MHC genotyping results obtained by deep sequencing of short multiplex PCR. 23 amplicons were only ...

MHC Genotyping in Human and Nonhuman Species by PCRbased ...

Recently, genotyping of the polymorphisms of MHC genes using targeted next-generation sequencing (NGS) technologies was developed for humans and some nonhuman ...

Stepwise Threshold Clustering: A New Method for Genotyping MHC ...

Stepwise Threshold Clustering: A New Method for Genotyping MHC Loci Using Next-Generation Sequencing Technology. William E. Stutz, Daniel I. Bolnick. William ...

Large-scale MHC class II genotyping of a wild lemur population by ...

The recent introduction of Next Generation Sequencing (NGS) to MHC genotyping, in contrast, shows promise to circumvent these problems. Indeed, ...

OptiType: precision HLA typing from next-generation sequencing data

One of the most recent approaches, published by Kim et al., uses a tree-based top-down greedy algorithm (HLAforest) to predict the HLA genotypes based on RNA- ...

HLA Sequencing | HLA typing with NGS - Illumina

HLA typing by next-generation sequencing (NGS) generates unambiguous, phase-resolved HLA typing results using a single assay, system, and analysis program.

Minimum information for reporting next generation sequence ...

... genotyping (MIRING): Guidelines for reporting HLA and KIR genotyping via next generation sequencing ... Any method for genotyping HLA and KIR using genomic DNA ...