Mathematical model of real|time PCR kinetics.

Mathematical model of real-time PCR kinetics - PubMed

We have developed a mathematical model of the annealing and extension phases of the PCR process. Using the model, we can simulate the PCR process over a series ...

Mathematical model of real-time PCR kinetics - Gene-Quantification

Mathematical Model of Real-Time. PCR Kinetics. Jana L. Gevertz,1 Stanley M. Dunn,1 Charles M. Roth1,2. 1Department of Biomedical Engineering, Rutgers ...

Mathematical model of real‐time PCR kinetics - Wiley Online Library

Abstract Several real-time PCR (rtPCR) quantification techniques are currently used to determine the expression levels of individual genes ...

Mathematical model of real-time PCR kinetics. - Semantic Scholar

A mathematical model of the annealing and extension phases of the PCR process is developed that allows us to predict the efficiency of rtPCR at any cycle ...

Validation of a quantitative method for real time PCR kinetics

Real time RT-PCR is the most sensitive method for quantitation of gene expression levels. The accuracy can be dependent on the mathematical model on which ...

Mathematical Model of Real-Time PCR Kinetics | Request PDF

A study proposed a mathematical model of PCR kinetics curve, they simulated the PCR process over a series of reaction cycles which allows for predicting the ...

Modeling Real-Time PCR Kinetics: Richards Reparametrized ...

Abstract ... Real-time PCR is the most sensitive method for detection and precise quantification of specific DNA sequences, but it is not usually applied as a ...

Mathematical Modeling of PCR with Variable Concentrations of DNA ...

Springer. 7. Gevertz, J.L., S.M. Dunn, and C.M. Roth, Mathematical model of real‐time PCR kinetics. Biotechnology and bioengineering, 2005 ...

Mathematics of quantitative kinetic PCR and the application of ...

Kinetic PCR (kPCR) allows quantification of a target DNA within a sample, with the advantage that sensitivity is independent of copy number (1–4). The key ...

A new mathematical model for relative quantification in real-time RT ...

Use of the real-time polymerase chain reaction (PCR) to amplify cDNA products reverse transcribed from. mRNA is on the way to becoming a routine tool in.

A kinetic-based sigmoidal model for the polymerase chain reaction ...

Based upon defining a common reference point, current real-time quantitative PCR technologies compare relative differences in amplification ...

Validation of a quantitative method for real time PCR kinetics

The generally accepted mathematical model assumes that amplification efficiencies are equal at the exponential phase of the reactions for the same amplicon.

Real-Time PCR for mRNA Quantitation - Taylor & Francis Online

A new mathematical model for relative quantification in real-time RT-PCR. ... Validation of a quantitative method for real time PCR kinetics. Biochem ...

Standardized determination of real‐time PCR efficiency from a ...

... method of amplification efficiency determination that uses the reaction kinetics ... ) A new mathematical model for relative quantification in real‐time RT–PCR.

Mathematical Modeling of PCR with Variable Concentrations of DNA ...

Gevertz, J.L., S.M. Dunn, and C.M. Roth, Mathematical model of real-time PCR kinetics. Biotechnol Bioeng , 2005. 92(3): p. 346–55.

Mathematical modeling of PCR amplification. In order to examine the...

It is based on the statistical significance of the beginning of exponential phase in real-time PCR kinetics, reflecting quantitative cycle of the initial target ...

Analysis of PCR Kinetics inside a Microfluidic DNA Amplification ...

... mathematical models of PCR kinetics. Then kinetic equations ... Mehra, S.; Hu, W.S. A kinetic model of quantitative real-time polymerase chain reaction.

Mathematical model of polymerase chain reaction with temperature ...

The course of the real-time polymerase chain reaction (PCR) is determined by the temperature dependence of the kinetics of the component ...

A new real-time PCR method to overcome significant quantitative ...

Gevertz JL, Dunn SM, Roth CM: Mathematical model of real-time PCR kinetics. Biotechnol Bioeng 2005, 92(3):346–355. 10.1002/bit.20617. Article ...

[PDF] Validation of a quantitative method for real time PCR kinetics.

A new mathematical model for relative quantification in real-time RT-PCR. M. Pfaffl. Mathematics, Biology. Nucleic acids research. 2001. TLDR. This study enters ...