Measure 2 Photon Action Cross Sections of Protein Bound ...

Used a novel measurement technique developed in this laboratory to determine the two-photon uncaging action cross section of DMNPE-4, a new calcium cage ...

Two-photon absorption properties of fluorescent proteins - PMC

The 2PA spectrum (presented in absolute cross section values per mature chromophore) represents the fundamental molecular property of a ...

Measurements of multiphoton action cross sections for ... - NCBI

Multiphoton microscopy (MPM) has several advantages in in vivo imaging of biological tissues. Because two- or three-photon excited fluorescence ...

Determination of Two-Photon-Absorption Cross Sections Using ...

Application of fluorescent proteins (FPs), e.g., as probes for biological imaging, has led to the goal of finding FPs with notable one- and ...

Two-Photon Excitation Spectra of Various Fluorescent Proteins ...

The probability of a two-photon absorption process of a fluorescent molecule is given by the two-photon action cross-section σ2PEη2PE, the ...

Two-photon fluorescence excitation cross sections of biomolecular ...

There are two ways of determining the two-photon cross section of a molecular fluorophore. One way is to measure the TPA cross section. This is done by the ...

Impact of the Protein Environment on Two-Photon Absorption Cross ...

The search for fluorescent proteins with large two-photon absorption (TPA) cross-sections and improved brightness is required for their ...

Two-photon excitation action cross-section of the autofluorescent ...

The definition of a relative detection yield between the autofluorescent proteins and flavine allows us to quantify the applicability of autofluorescent ...

Two-Photon Fluorescence Spectroscopy and Microscopy of NAD(P ...

In contrast, 2P-FP fluorescence dominates at 900-nm excitation, which is in agreement with the σ2P measurements. Finally, 2P-autofluorescence emission spectra ...

More than double the fun with two-photon excitation microscopy

It is worth noting that the efficiency of two-photon absorption (action cross section of the fluorophore in units of GM), a molecular ...

(PDF) Measurements of multiphoton action cross sections for ...

We report quantitative measurements of two-, three-, and four-photon excitation action cross sections of several commonly used fluorophores ...

Two-photon excitation microscopy of tryptophan-containing proteins

Two-dimensional images were acquired by scanning the sample through the focus. The image acquisition was controlled by a photon counter (SR400, Stanford ...

A new approach to dual-color two-photon microscopy with ...

Two-photon dual-color imaging of tissues and cells labeled with fluorescent proteins (FPs) is challenging because most two-photon ...

Multiphoton excitation cross-sections of molecular fluorophores

Two-photon excitation cross section measurements ... two-photon action cross sections except for ... 2 a DAPI not bound to DNA. The ...

Two-Photon Activation and Excitation Properties of PA-GFP in the ...

... 2 / 2 ℏ c λ ) 2 ) (12). For ... Measurement of dynamic protein binding to ... Two-photon excitation action cross-sections of the autofluorescent proteins.

Multiphoton-excited luminescence of a lanthanide ion in a protein ...

d Two-photon action cross-section. Table 2 Comparison of luminescence intensities determined for Tb2–Tfr (100 µM at pH 9) after two-photon excitation at λex = ...

Two-Photon Microscopy of Cells and Tissue | Circulation Research

... measurements by two-photon ... protein binding or pH changes. Green ... Toward this end, new cages with larger two-photon absorption action cross sections ...

Two-photon excitation action cross-sections of the autofluorescent ...

Concentrations of the fluorescent proteins were determined by measuring their absorption spectra. SDS-PAGE analysis revealed a correct molecular ...

Measurement of three-photon excitation cross-sections of ...

The three-photon action cross-section at 1154 nm is more than an order of magnitude larger than those at 1450 and 1500 nm (approximately three ...

Two-Photon Microscopy of Cells and Tissue - AHA Journals

determine the TPE action cross section are technically de- ... Measurement of two-photon excitation cross sections ... Intravital imaging of green fluorescent ...