- Mechanical and chemical unfolding of a single protein🔍
- Single|molecule chemo|mechanical unfolding reveals multiple ...🔍
- Mechanical unfolding studies of protein molecules🔍
- Mechanical Unfolding Response of Proteins🔍
- Mechanical design of proteins studied by single|molecule force ...🔍
- Protein Folding Under Mechanical Forces🔍
- The nanomechanics of individual proteins🔍
- Compliant mechanical response of the ultrafast folding protein ...🔍
Mechanical and chemical unfolding of a single protein
Mechanical and chemical unfolding of a single protein: A comparison
The native state of proteins is the most stable, and therefore, proteins rarely unfold spontaneously. For example, the unfolding of isolated Ig and fibronectin ...
Mechanical and chemical unfolding of a single protein - PubMed
Is the mechanical unraveling of protein domains by atomic force microscopy (AFM) just a technological feat or a true measurement of their unfolding?
Mechanical and chemical unfolding of a single protein: A comparison
Furthermore, the transition state for unfolding appears at the same position on the folding pathway when assessed by either method. These results indicate that ...
Mechanical and chemical unfolding of a single protein: A comparison
These results indicate that mechanical unfolding of a single protein by AFM does indeed reflect the same event that is observed in traditional ...
Single-molecule chemo-mechanical unfolding reveals multiple ...
A fundamental question in protein folding is whether proteins fold through one or multiple trajectories. While most experiments indicate a ...
Mechanical and chemical unfolding of a single protein - ResearchGate
These results indicate that mechanical unfolding of a single protein by AFM does indeed reflect the same event that is observed in traditional unfolding ...
Mechanical unfolding studies of protein molecules - PMC
Atomic force microscopy (AFM) enables the pick up of a single protein molecule to apply a mechanical force. This technique, called “force spectroscopy,” ...
Mechanical and chemical unfolding of a single protein: A comparison
By engineering a protein made of tandem repeats of identical Ig modules, we were able to get explicit AFM data on the unfolding rate of a single protein domain ...
Mechanical Unfolding Response of Proteins | ACS In Focus
Mechanical Unfolding Response of Proteins · Bioengineering and biotechnology · Biology · Biophysics · Biopolymers · Chemical engineering and ...
Mechanical design of proteins studied by single-molecule force ...
Mechanical unfolding and refolding may regulate the molecular elasticity of modular proteins with mechanical functions. The development of the atomic force ...
Protein Folding Under Mechanical Forces: A Physiological View
Protein unfolding requires the protein to overcome one or more energy barriers that result from the breaking of hydrogen bonds. Only this energy barrier is ...
The nanomechanics of individual proteins - RSC Publishing
Yet, the molecular mechanisms controlling the mechanical unfolding and refolding of proteins cannot be accessed by protein folding biochemistry ...
(PDF) Me22 chanical and chemical unfolding of a single protein
Using single protein atomic force microscopy (AFM) techniques we demonstrate that after repeated mechanical extension/relaxation cycles, tandem modular proteins ...
Compliant mechanical response of the ultrafast folding protein ...
However, single molecule force spectroscopy experiments on these systems are challenging as these proteins do not provide the mechanical ...
Reversible Mechanical Unfolding of Single Ubiquitin Molecules
Experimental evidence suggests that force-induced protein unfolding is an essential step in several important cellular processes, such as protein degradation by ...
Chemical Protein Unfolding – A Simple Cooperative Model
For a given denaturant, the binding constant depends on temperature and pH but shows little protein specificity. Chemical unfolding is less ...
Harvesting Mechanical Work From Folding-Based Protein Engines
Here, the authors demonstrate that protein folding can be utilized to realize protein's mechanochemical cycles at both single-molecule and ...
How do chemical denaturants affect the mechanical folding and ...
This study demonstrates great potential in combining chemical denaturation with single-molecule atomic force microscopy techniques to reveal invaluable ...
Mechanically Unfolding the Small, Topologically Simple Protein L
β-sheet proteins are generally more able to resist mechanical deformation than α-helical proteins. Experiments measuring the mechanical resistance of ...
History, rare, and multiple events of mechanical unfolding of repeat ...
Mechanical unfolding of proteins consisting of repeat domains is an excellent tool to obtain large statistics.