Metabolic Flux Analysis and In Vivo Isotope Tracing

Tracing metabolic flux in vivo: basic model structures of tracer ...

Metabolic flux analysis (MFA) with the use of stable isotope tracers is an approach to approximate the flux rates of components of metabolic ...

Metabolic Flux Analysis and In Vivo Isotope Tracing ... Metabolic flux is the dynamic process of tracking the changes in metabolite levels throughout the entire ...

Measurement of metabolic fluxes using stable isotope tracers in ...

Metabolic flux analysis using stable isotope labeled substrates allows for the tracing ... METABOLIC FLUX ANALYSIS USING STABLE ISOTOPE LABELS IN VIVO. A critical ...

Global 13C tracing and metabolic flux analysis of intact human liver ...

... vivo using global 13C tracing, non-targeted mass spectrometry and model-based metabolic flux analysis. Isotope tracing allowed qualitative ...

Tracing metabolic flux in vivo: motion pictures differ from snapshots

Wolfe, R. R. & Chinkes, D. L. Isotope Tracers in Metabolic Research: Principles and Practice of Kinetic Analysis (Wiley-Liss, 2005). 6. Kim IY, ...

Isotope tracing in health and disease - ScienceDirect.com

For metabolic flux analysis, isotopic steady states should be experimentally validated before computational analysis. Last, for in vivo tracing ...

Basics of metabolite profiling and metabolic flux analysis

Isotopic tracing to study the activity of metabolic pathways. • Consider tracer uptake (e.g., glucose uptake) before performing the tracing.

Tracing metabolic flux through time and space with isotope labeling ...

Quantifying plant phenotypes with isotopic labeling & metabolic flux analysis ... metabolic fluxes in vivo. Am J Physiol Endocrinol Metab ...

One‐shot 13C15N‐metabolic flux analysis for simultaneous ...

... fluxes, isotope tracing studies in combination with computational modeling is a gold standard. Recently, multiple stable isotopic tracers ...

Applications of Stable Isotope Labeling in Metabolic Flux Analysis

Metabolic Flux Analysis (MFA) Service. Learn more. Metabolic Flux Analysis: Unraveling Cellular Metabolism · Metabolic Flux Analysis and In Vivo Isotope Tracing ...

13 C metabolic flux analysis: Classification and characterization ...

... fluxomics, an isotope-labeled tracer is incorporated into the metabolic ... isotope ratio mass spectrometry analysis for in vivo estimates of metabolic fluxes.

Metabolic flux analysis: a comprehensive review on sample ...

During isotope incorporation, the 13C tracer is quickly distributed all over the metabolic pathways until the isotopic steady state is reached ...

In vivo isotope tracing reveals the versatility of glucose as a brown ...

Figure 2 Integrative transcriptomics and metabolic tracing analysis reveal increased BAT glycolytic flux upon SC. Show full captionFigure viewer.

Metabolic Flux Analysis - Sciex

Metabolic flux analysis holds the key to observing the true dynamics of a system. Stable isotope labeling for LC-MS analysis is one of the primary approaches.

In Vivo and In Vitro Quantification of Glucose Kinetics

In addition, assessments of metabolic flux using stable isotope tracer ... 13C tracing in metabolic network in vitro using metabolic flux analysis ...

In Vivo Estimates of Liver Metabolic Flux Assessed by 13C ...

Discussion. Isotope-based flux analysis is the gold standard for measurements of in vivo metabolism and has provided important insights into ...

Isotopically nonstationary metabolic flux analysis of plants: recent ...

Historical descriptions of photosynthetic, central, and lipid metabolic pathways are based on isotope tracing (Allen et al., 2015); however, MFA ...

Global 13C tracing and metabolic flux analysis of intact human liver ...

Isotope tracing ex vivo allows measuring human liver metabolism with great depth and resolution in an experimentally tractable system. The ...

Stable Isotopes for Tracing Cardiac Metabolism in Diseases - Frontiers

... flux through the hexosamine biosynthesis pathway (HBP) in ex vivo ... Keywords: metabolism, stable-isotope tracer, metabolic flux analysis, systems biology, ...

Extracellular Flux Analysis and 13C Stable-Isotope Tracing ... - Agilent

Eukaryotic cells are known to generate ATP by mitochondrial (oxidative phosphorylation) and non-mitochondrial (glycolysis) metabolism.