Methods for MHC genotyping in non‐model vertebrates

Methods for MHC genotyping in non-model vertebrates - PubMed

Here, I discuss the DNA-based methods currently used for genotyping MHC in non-model vertebrates, as well as techniques likely to find widespread use in the ...

Methods for MHC genotyping in non‐model vertebrates - BABIK - 2010

Here, I discuss the DNA-based methods currently used for genotyping MHC in non-model vertebrates, as well as techniques likely to find widespread use in the ...

Methods for MHC genotyping in non‐model vertebrates - BABIK - 2010

Accurate genotyping is essential for understanding of the role that MHC variation plays in natural populations, but may be extremely challenging ...

Methods for MHC genotyping in non‐model vertebrates | Semantic ...

This review addresses the well established typing methods such as the Single Strand Conformation Polymorphism (SSCP), Denaturing Gradient Gel ...

Improved high‐throughput MHC typing for non‐model species using ...

Despite the recent expansion in next‐generation sequencing methods, Sanger sequencing (cycle sequencing) remains the most commonly used MHC typing method (43% ...

Babik W.. Methods for MHC genotyping in non-model vertebrates ...

Alleles were defined on the basis of the standard criterion in MHC genotyping (Babik 2010) : sequences are considered to be valid alleles only when they are ...

MHC genotyping of non-model organisms using next-generation ...

Correct assessment of the individual allelic diversity pattern and the underlying structural sequence variation is the basic requirement to ...

Using de novo genome assembly and high-throughput sequencing ...

Because of its complex architecture with tandems of duplicated genes, characterization of MHC region in non-model vertebrate species still poses ...

Methods for MHC genotyping in non-model vertebrates. (2010 ...

DOI: 10.1111/J.1755-0998.2009.02788.X) Genes of the major histocompatibility complex (MHC) are considered a paradigm of adaptive evolution at the molecular ...

Evolution of innate and adaptive immune genes in a non-model ...

Recent advancements in sequencing technology sparked research on highly duplicated MHC genes in non-model species, but TLR variation in natural vertebrate ...

A new hybrid approach for MHC genotyping: high-throughput NGS ...

... non-model vertebrate Alpine chamois (Rupicapra rupicapra) | Welcome to Oxford Nanopore technologies. Our goal is to enable the analysis of ...

Methods for MHC genotyping in non‐model vertebrates | CoLab

Genes of the major histocompatibility complex (MHC) are considered a paradigm of adaptive evolution at the molecular level and as such are frequently ...

A new hybrid approach for MHC genotyping: high-throughput NGS ...

A new hybrid approach for MHC genotyping: high-throughput NGS and long read MinION nanopore sequencing, with application to the non-model ...

MHC class IIa haplotypes derived by high-throughput SNP ...

Developing genotyping methods which attribute alleles to loci and loci to haplotypes are key technical challenges when studying the MHC in nonmodel species ( ...

MHC genotyping by SSCP and Amplicon-Based NGS Approach in ...

Methods for MHC genotyping in non-model vertebrates. Mol. Ecol. Resour. 2010, 10, 237–251. [CrossRef]. 18. Sunnucks, P.; Wilson, A.C.C. ...

Genotyping MHC classIIB in non-model species by reference strand ...

For genotyping of individual fish, MHC-genes were amplified with the ... Babik W (2009) Methods for MHC genotyping in non model vertebrates, Mol Ecol ...

MHC Genotyping in Human and Nonhuman Species by PCRbased ...

Most species have numerous highly homologous MHC loci so the NGS technologies are likely to replace traditional genotyping methods in the near future for the ...

Performance Comparison of Different Approaches in Genotyping ...

Major histocompatibility complex (MHC) genes are widely recognised as valuable markers for wildlife genetic studies given their extreme polymorphism and ...

Large-scale MHC class II genotyping of a wild lemur population by ...

The quality of our genotyping was evaluated through three independent methods, namely genotyping the same individuals by both cloning and 454 ...

Next-generation genotyping of hypervariable loci in many ...

We demonstrate the utility of using NGS to genotype non-model organisms at complex hypervariable loci like those of the MHC and we validate the ...